A simple method to determine the immunoreactivity of radiolabelled monoclonal antibodies to the TAG-72 antigen

A simple method has been developed for determining the immunoreactivity of radiolabelled monoclonal antibodies to the TAG-72 antigen. The method involves binding of a constant small amount of the antibody to increasing concentrations of bovine submaxillary mucin. The immunoreactive fraction (IRF) is...

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Bibliographic Details
Published in:Applied radiation and isotopes 1993, Vol.44 (9), p.1193-1197
Main Authors: Ngai, W.M., Reilly, R.M.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glycoproteins - immunology
Humans
Iodine Radioisotopes
Molecular immunology
Radioimmunodetection - methods
Techniques
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Summary:A simple method has been developed for determining the immunoreactivity of radiolabelled monoclonal antibodies to the TAG-72 antigen. The method involves binding of a constant small amount of the antibody to increasing concentrations of bovine submaxillary mucin. The immunoreactive fraction (IRF) is then determined by linear extrapolation of binding to infinite antigen excess. Using this assay, the IRF of radioiodinated anti-TAG-72 antibodies ranged from 0.22–0.48.
ISSN:0969-8043
1872-9800
DOI:10.1016/0969-8043(93)90063-G