Mouse Model of Arterial Injury

In the present study, we established an injury model of the mouse carotid artery. Complete removal of the endothelium was achieved with a flexible wire. A platelet monolayer covered the denuded surface, and damage to underlying medial smooth muscle cells (SMCs) was detected. Injection of [H]thymidin...

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Bibliographic Details
Published in:Circulation research 1993-11, Vol.73 (5), p.792-796
Main Authors: Lindner, Volkhard, Fingerle, Jürgen, Reidy, Michael A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carotid Arteries - pathology
Cell Division
Disease Models, Animal
Endothelium, Vascular - physiology
Female
Injuries of the nervous system and the skull. Diseases due to physical agents
Medical sciences
Mice
Muscle, Smooth, Vascular - pathology
Traumas. Diseases due to physical agents
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Summary:In the present study, we established an injury model of the mouse carotid artery. Complete removal of the endothelium was achieved with a flexible wire. A platelet monolayer covered the denuded surface, and damage to underlying medial smooth muscle cells (SMCs) was detected. Injection of [H]thymidine was used to determine the replication index for medial SMCs, which was found to be 1.6% at 2 days after denudation and 9.8% at 5 days. SMCs were observed in the intima by day 8 (replication index, 66%), and by 2 weeks the intimal lesion had a similar cell content as the media. In most animals, repair of the endothelial lining was complete 3 weeks after injury. The present model will allow us to use transgenic animals to address questions relevant to vascular biology and atherosclerosis.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.73.5.792