The synthesis of α 2-macroglobulin by rat mammary myoepithelial cells is regulated by synergism between glucocorticoids and cytokines

Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of α 2-macroglobulin. Interleukin-1β, interleukin-6 and transforming growth factor-β synergise with hydrocortisone, increasing the synthesis of α 2-macroglobulin 2- to 4-fold, alt...

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Bibliographic Details
Published in:FEBS letters 1993-10, Vol.332 (1), p.57-60
Main Authors: Warburton, Michael J., Gusterson, Barry A., O'Hare, Michael J.
Format: Article
Language:English
Subjects:
alpha-Macroglobulins - biosynthesis
Animals
Cell Line
Cytokines - physiology
Epithelium - metabolism
Hydrocortisone - physiology
Interleukin-1 - physiology
Interleukin-6
Interleukin-6 - physiology
Mammary gland
Mammary Glands, Animal - metabolism
Myoepithelial cell
Rats
Transforming Growth Factor beta - physiology
α 2-Macroglobulin
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Summary:Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of α 2-macroglobulin. Interleukin-1β, interleukin-6 and transforming growth factor-β synergise with hydrocortisone, increasing the synthesis of α 2-macroglobulin 2- to 4-fold, although they have no effect in the absence of hydrocortisone. Interleukin-6 is the most potent inducer having an optimum concentration of 1 ng ml . Interleukin-6, unlike interleukin-1β or tranforming growth factor-β, decreases the lag phase from 10 h to 4 h before α 2-macroglobulin synthesis is induced by hydrocortisone. These results suggest that the mechanism of activation of transcription of the α 2-macroglobulin gene in mammary myoepithelial cells might differ from that operating in hepatic cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80483-B