Localization of amidating enzymes (PAM) in rat gastrointestinal tract

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric a...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1993-11, Vol.41 (11), p.1617-1622
Main Authors: Martinez, A, Burrell, MA, Kuijk, M, Montuenga, LM, Treston, A, Cuttitta, F, Polak, JM
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Colon - enzymology
Colon - innervation
Endocrine Glands - enzymology
Fundamental and applied biological sciences. Psychology
Glucagon - metabolism
Glucagon-Like Peptide 1
Immunoenzyme Techniques
In Situ Hybridization
Intestine, Small - enzymology
Intestine, Small - innervation
Intestine. Mesentery
Mixed Function Oxygenases - metabolism
Multienzyme Complexes
Nerve Fibers - enzymology
Neurons - enzymology
Peptide Fragments - metabolism
Protein Precursors - metabolism
Rats
Rats, Wistar
Stomach - enzymology
Stomach - innervation
Vertebrates: digestive system
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Summary:We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.
ISSN:0022-1554
1551-5044
DOI:10.1177/41.11.8409369