Beta adrenergic and muscarinic receptors in compensatory cardiac hypertrophy of the adult rat

• Published in: Pflügers Archiv 1993-08, Vol.424 (3-4), p.354-360
• Main Authors: MANSIER, P, CHEVALIER, B, BARNETT, D. B, SWYNGHEDAUW, B
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclases - metabolism; Animals; Aortic Valve Stenosis - physiopathology; Biological and medical sciences; Body Weight - drug effects; Carbachol - pharmacology; Cardiology. Vascular system; Cyclic AMP - metabolism; Heart; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; Hypertrophy, Left Ventricular - metabolism; In Vitro Techniques; Iodine Radioisotopes; Isoproterenol - pharmacokinetics; Kinetics; Male; Medical sciences; Membrane Proteins - metabolism; Organ Size - drug effects; Quinuclidinyl Benzilate - pharmacokinetics; Rats; Rats, Wistar; Receptors, Adrenergic, beta - metabolism; Receptors, Adrenergic, beta-1 - metabolism; Receptors, Adrenergic, beta-2 - metabolism; Receptors, Muscarinic - metabolism
• ISSN: 0031-6768; 1432-2013
• DOI: 10.1007/BF00384363

The beta adrenergic (beta AR) and muscarinic (MR) receptors have been quantitated in parallel, using 125I-pindolol and 3H-quinuclidinylbenzilate, in a model of compensatory left ventricular (LV) hypertrophy (LVH), which developed in rats 4-6 weeks after an abdominal aortic stenosis. Since aortic banding resulted in a pronounced LVH of 62%, the results were expressed both in terms of density (fmol/mg protein) and quantity (fmol per LV). In addition, competition curves using either a specific beta 1-antagonist or isoproterenol or carbachol allowed the determination of the two beta AR subtypes and of the low and high affinity sites (defined by the inhibitory constant Ki) for both beta 1AR and MR. In LVH, receptor density decreased for each of total beta AR, beta 1AR subtype, high affinity (Ki 6-8 nM) beta 1AR sites (from 26 +/- 2 to 19 +/- 3 fmol/mg protein, P < 0.05), total MR and high affinity (Ki 12 nM) MR sites (from 63 +/- 6 to 40 +/- 4 fmol/mg protein, P < 0.001). The beta AR and MR densities dropped in parallel so that the MR/beta AR ratio remained unchanged. In sharp contrast (because the LVs were bigger) the quantities of total beta AR, beta 1AR subtype, beta 1AR high affinity sites, total MR and MR high affinity sites per LV were unmodified.