Antigenic Characterization of Respiratory Syncytial Virus Strains with Monoclonal Antibodies

To study the antigenic characteristics of respiratory syncytial virus (RSV), we developed and evaluated monoclonal antibodies (MAbs) to three strains of RSV: 11 to Long, 4 to 18537, and 9 to A2. Six of these MAbs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion pro...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1985-04, Vol.151 (4), p.626-633
Main Authors: Anderson, Larry J., Hierholzer, John C., Tsou, Cecilia, Hendry, R. Michael, Fernie, Bruce F., Stone, Yvonne, McIntosh, Kenneth
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigens, Viral - immunology
Biological and medical sciences
Enzyme linked immunosorbent assay
Epidemiology
Epitopes
Epitopes - immunology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Glycoproteins - immunology
Human respiratory syncytial virus
Microbiology
Monoclonal antibodies
Nucleoproteins - immunology
Original Articles
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Respiratory syncytial viruses
Respiratory Syncytial Viruses - classification
Respiratory Syncytial Viruses - immunology
Serotyping
Species Specificity
Test systems
Viral Envelope Proteins - immunology
Viral Fusion Proteins
Viral proteins
Viral Proteins - immunology
Virology
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To study the antigenic characteristics of respiratory syncytial virus (RSV), we developed and evaluated monoclonal antibodies (MAbs) to three strains of RSV: 11 to Long, 4 to 18537, and 9 to A2. Six of these MAbs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. By the pattern of the reactions of these MAbs to 16 strains of RSV in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. With a panel of 10 of these 24 MAbs, we tested 26 strains isolated between 1979 and 1982 in Boston and found that 22 belonged to group 1, 4 to group 2, and none to group 3. The pattern of the reactions of the MAbs against representative strains from the three groups identified nine epitopes by indirect immunofluorescence assay: three of each on the nucleoprotein, the large glycoprotein, and the fusion protein. These results, along with those of previous studies, suggest that groups 1 and 3 are antigenically similar and group 2 is antigenically more distinct.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/151.4.626