Biosynthesis of vitamin B12 : discovery of the enzymes for oxidative ring contraction and insertion of the fourth methyl group

In the vitamin B12 biosynthetic pathway the enzymes responsible for the conversion of precorrin-3 to precorrin-4 have been identified as the gene products of cobG and cobJ from Pseudomonas denitrificans. CobG catalyzes the oxidation of precorrin-3 to precorrin-3x (a hydroxy lactone) whereas CobJ is...

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Bibliographic Details
Published in:FEBS letters 1993-09, Vol.331 (1-2), p.105-108
Main Authors: SCOTT, A. I, ROESSNER, C. A, STOLOWICH, N. J, SPENCER, J. B, MIN, C, OZAKI, S.-I
Format: Article
Language:English
Subjects:
Bacterial Proteins
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Single-Stranded
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Methylation
Methyltransferases - genetics
Methyltransferases - metabolism
Microbiology
Molecular Sequence Data
Oxidation-Reduction
Oxygenases - genetics
Oxygenases - metabolism
Pseudomonas - enzymology
S-Adenosylmethionine - metabolism
Uroporphyrins - biosynthesis
Vitamin B 12 - biosynthesis
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Summary:In the vitamin B12 biosynthetic pathway the enzymes responsible for the conversion of precorrin-3 to precorrin-4 have been identified as the gene products of cobG and cobJ from Pseudomonas denitrificans. CobG catalyzes the oxidation of precorrin-3 to precorrin-3x (a hydroxy lactone) whereas CobJ is a SAM-dependent C-17 methyl transferase and is necessary for ring contraction. A mechanism for ring contraction is proposed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80306-F