Crystal structure of a yeast TBP/TATA-box complex

The 2.5 angstrom crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primar...

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Bibliographic Details
Published in:Nature (London) 1993-10, Vol.365 (6446), p.512-520
Main Authors: Kim, Y, Geiger, J.H, Hahn, S, Sigler, P.B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biochemistry
Biological and medical sciences
Computer Graphics
Crystallography, X-Ray
Cytochrome c Group - genetics
Cytochromes c
DNA
DNA - chemistry
Fundamental and applied biological sciences. Psychology
genetic techniques and protocols
Genetics
Humanities and Social Sciences
Hydrogen Bonding
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
multidisciplinary
Nucleic Acid Conformation
Promoter Regions, Genetic
Protein Binding
Protein Conformation
Proteins
Recombinant Proteins - chemistry
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae Proteins
Science
Science (multidisciplinary)
Structure-Activity Relationship
TATA Box
Transcription Factor TFIIA
Transcription Factor TFIID
Transcription Factors - chemistry
Transcription Factors - metabolism
Transcription. Transcription factor. Splicing. Rna processing
Yeast
Yeasts
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Summary:The 2.5 angstrom crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.
ISSN:0028-0836
1476-4687
DOI:10.1038/365512a0