Studies on the Ability of Alginate to Act as a Protective Immunogen Against Infection with Pseudomonas aeruginosa in Animals

Most patients with cystic fibrosis become colonized and infected with mucoid Pseudomonas aeruginosa. The major component of the mucoid material has been identified as the polysaccharide alginic acid. The present work was undertaken to determine whether antibody to alginate is protective in a model o...

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Bibliographic Details
Published in:The Journal of infectious diseases 1985-04, Vol.151 (4), p.581-588
Main Authors: Woods, D. E., Bryan, L. E.
Format: Article
Language:English
Subjects:
Alginates
Alginates - immunology
Animals
Antibodies
Antibodies, Bacterial - analysis
Antibodies, Bacterial - biosynthesis
Antigen antibody complex
Antigen-Antibody Complex - analysis
Antigens
Bacterial diseases
Biological and medical sciences
Bronchi - pathology
Cystic fibrosis
Experimental bacterial diseases and models
Fluorescence
Fluorescent Antibody Technique
Immunization
Infections
Infectious diseases
Lung - immunology
Lung - pathology
Lung diseases
Lung Diseases - immunology
Lung Diseases - microbiology
Lung Diseases - pathology
Lungs
Lymph Nodes - pathology
Male
Medical sciences
Original Articles
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas Infections - immunology
Pseudomonas Infections - microbiology
Pseudomonas Infections - pathology
Pulmonary Alveoli - immunology
Rats
Rats, Inbred Strains
Citations: Items that cite this one
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Description
Summary:Most patients with cystic fibrosis become colonized and infected with mucoid Pseudomonas aeruginosa. The major component of the mucoid material has been identified as the polysaccharide alginic acid. The present work was undertaken to determine whether antibody to alginate is protective in a model of chronic lung infection with P. aeruginosain rats. Bacterial clearance was associated with a rise in titers of antibody to alginate. In a number of animals a rise in antibody titers was not seen, and in fact a decrease was noted at 30 days compared with 10 days. This observation suggested the possibility of immune complex formation due to antigen excess. Evidence for immune complex deposition in tissues was obtained by immunofluorescence studies. Thus antibody to alginate may offer strain-dependent protection against chronic lung infection with P. aeruginosa in rats; however, immune complex formation should be considered as a possible consequence of immunization with alginate.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/151.4.581