Flow Effects on Prostacyclin Production by Cultured Human Endothelial Cells

Endothelial cell functions, such as arachidonic acid metabolism, may be modulated by membrane stresses induced by blood flow. The production of prostacyclin by primary human endothelial cell cultures subjected to pulsatile and steady flow shear stress was measured. The onset of flow led to a sudden...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1985-03, Vol.227 (4693), p.1477-1479
Main Authors: Frangos, John A., Eskin, Suzanne G., McIntire, Larry V., Ives, C. L.
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
Blood Circulation
Blood flow
Cell metabolism, cell oxidation
Cell physiology
Cell research
Cells, Cultured
Chemical engineering
Cultured cells
Cytological research
Endothelial cells
Endothelium
Endothelium - cytology
Endothelium - metabolism
Epoprostenol - biosynthesis
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Methods. Procedures. Technologies
Miscellaneous
Models, Biological
Molecular and cellular biology
Oscillators
Parallel plates
Physiological aspects
Prostacyclin
Pumps
Shear stress
Space life sciences
Steady flows
Stress, Mechanical
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Summary:Endothelial cell functions, such as arachidonic acid metabolism, may be modulated by membrane stresses induced by blood flow. The production of prostacyclin by primary human endothelial cell cultures subjected to pulsatile and steady flow shear stress was measured. The onset of flow led to a sudden increase in prostacyclin production, which decreased to a steady rate within several minutes. The steady-state production rate of cells subjected to pulsatile shear stress was more than twice that of cells exposed to steady shear stress and 16 times greater than that of cells in stationary culture.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3883488