Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells

A UV-sensitive mutant line of CHO cells, UV20, was shown to be phenotypically corrected to resistance by fusion with human lymphocytes or fibroblasts. Only human chromosome 19 correlated with the DNA repair phenotype of resistant hybrid clones and their resistant or sensitive subclones. This study d...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1985-01, Vol.11 (1), p.87-92
Main Authors: Thompson, L H, Mooney, C L, Burkhart-Schultz, K, Carrano, A V, Siciliano, M J
Format: Article
Language:English
Subjects:
Animals
Chromosomes, Human, 19-20
Cricetinae
Cricetulus
DNA Repair - radiation effects
Drug Resistance
Humans
Hybrid Cells - metabolism
Mitomycin
Mitomycins - pharmacology
Mutation
Ultraviolet Rays
Xeroderma Pigmentosum - genetics
Xeroderma Pigmentosum - metabolism
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Summary:A UV-sensitive mutant line of CHO cells, UV20, was shown to be phenotypically corrected to resistance by fusion with human lymphocytes or fibroblasts. Only human chromosome 19 correlated with the DNA repair phenotype of resistant hybrid clones and their resistant or sensitive subclones. This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C).
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01534738