Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos

The demonstration that a specific messenger RNA can he functionally inactivated in vivo hy hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene function in cells of higher organisms 1,2 . The experiments described here were designed to inhibit, by c...

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Bibliographic Details
Published in:Nature (London) 1985-02, Vol.313 (6004), p.703-706
Main Authors: Rosenberg, Urs B, Preiss, Anette, Seifert, Eveline, Jäckle, Herbert, Knipple, Douglas C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Humanities and Social Sciences
letter
Microinjections
Molecular and cellular biology
multidisciplinary
Mutation
Phenotype
RNA - genetics
RNA, Messenger - genetics
Science
Science (multidisciplinary)
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Summary:The demonstration that a specific messenger RNA can he functionally inactivated in vivo hy hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene function in cells of higher organisms 1,2 . The experiments described here were designed to inhibit, by complementary RNA sequences, a specific gene function affecting the fate of the Drosophila embryo. We used the SP6 vector in vitro transcription system 3 to transcribe parts of the normally untranscribed (nonsense) strand of the Krüppel ( Kr ) gene into complementary Kr RNA ( Kr antisense RNA). Wild-type Drosophila embryos, injected with this RNA, developed into phenocopies of Kr mutant embryos.
ISSN:0028-0836
1476-4687
DOI:10.1038/313703a0