Physico-chemical conditions necessary for the in vitro excystment of Zygocotyle lunata (Trematoda: Paramphistomatidae)

Various physico-chemical factors associated with the in vitro excystment of metacercariae of Zygocotyle lunata were studied. Metacercariae could excyst with or without the presence of trypsin or bile salts, although the rate of excystation was slower in the absence of both. Crude ox-bile extract pro...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 1993, Vol.79 (5), p.416-420
Main Authors: IRWIN, S. W. B, O'KANE, M. B, FRIED, B
Format: Article
Language:English
Subjects:
Animals
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Invertebrates
Nemathelminthia. Plathelmintha
Paramphistomatidae - physiology
Physiology. Development
Salts
Snails - parasitology
Temperature
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various physico-chemical factors associated with the in vitro excystment of metacercariae of Zygocotyle lunata were studied. Metacercariae could excyst with or without the presence of trypsin or bile salts, although the rate of excystation was slower in the absence of both. Crude ox-bile extract promoted faster excystment than did more refined sodium taurocholate. Pretreatment of cysts with pepsin also resulted in faster excystment rates. Optimal excystation occurred at 40 degrees C and between pH 5.5 and 7.5. The broad pH and temperature ranges and the non-specific enzyme/bile salt requirements for excystation were correlated with the wide range of vertebrate hosts used by this trematode.
ISSN:0932-0113
DOI:10.1007/BF00931832