Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex

Several proteins are involved in the early steps of the spliceosome assembly pathway. Protein-protein interactions have been identified between two Saccharomyces cerevisiae yeast splicing factors, PRP9 and SPP91. Here it is demonstrated that protein-protein interactions occur between SPP91 and PRP11...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1993-10, Vol.262 (5130), p.108-110
Main Authors: Legrain, P, Chapon, C
Format: Article
Language:English
Subjects:
ARN MENSAJERO
ARN MESSAGER
Biological and medical sciences
Cells
DNA
Dominant lethals
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
GENE
GENES
Genes, Fungal
Genes, Reporter
Genetic mutation
GENIE BIOCHIMIQUE
INGENIERIA BIOQUIMICA
Molecular and cellular biology
Molecular genetics
MUTANT
MUTANTES
Mutation
Phenotype
Phenotypes
Plasmids
Recombinant Fusion Proteins - biosynthesis
RNA Splicing
RNA Splicing Factors
RNA-Binding Proteins
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Small nuclear ribonucleoproteins
Spliceosomes
Spliceosomes - metabolism
Splicing
Transcription. Transcription factor. Splicing. Rna processing
Yeasts
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Description
Summary:Several proteins are involved in the early steps of the spliceosome assembly pathway. Protein-protein interactions have been identified between two Saccharomyces cerevisiae yeast splicing factors, PRP9 and SPP91. Here it is demonstrated that protein-protein interactions occur between SPP91 and PRP11. The combination of the prp9-l mutant and a truncated prp 11 mutant exhibits a synthetic lethal phenotype, suggestive of a common biochemical defect. The PRP9 and PRP1 1 proteins do not interact directly, but the PRP9 and PRP11 molecules can simultaneously bind SPP91 to form a three-molecule complex. Structurally and functionally related proteins are found in mammalian cells and are associated in a single biochemical fraction. This strongly suggests that the PRP9-SPP91 -PRP1 1 complex is a key element of the splicing machinery
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8211114