Amperometric glucose biosensor with remarkable acid stability based on glucose oxidase entrapped in colloidal gold-modified carbon ionic liquid electrode

• Published in: Biosensors & bioelectronics 2010-08, Vol.25 (12), p.2675-2679
• Main Authors: Liu, Xiaoying, Zeng, Xiandong, Mai, Nannan, Liu, Yong, Kong, Bo, Li, Yonghong, Wei, Wanzhi, Luo, Shenglian
• Format: Article
• Language: English
• Subjects: Acid stability; Biological and medical sciences; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Carbon; Carbon ionic liquid electrode; Colloidal gold; Electrochemical Techniques; Enzymes, Immobilized; Fundamental and applied biological sciences. Psychology; Glucose - analysis; Glucose Oxidase; Gold Colloid; Graphite; Hydrogen-Ion Concentration; Ionic Liquids; Limit of Detection; Methods. Procedures. Technologies; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2010.04.045

A colloidal gold-modified carbon ionic liquid electrode was constructed by mixing colloidal gold-modified graphite powder with a solid room temperature ionic liquid n-octyl-pyridinium hexafluorophosphate (OPPF 6). Glucose oxidase (GOD) was entrapped in this composite matrix and maintained its bioactivity well and displayed excellent stability. The effect conditions of pH, applied potential and GOD loading were examined. Especially, the glucose oxidase entrapped in this carbon ionic liquid electrode fully retained its activity upon stressing in strongly acidic conditions (pH 2.0) for over one hour. The proposed biosensor responds to glucose linearly over concentration range of 5.0 × 10 −6 to 1.2 × 10 −3 and 2.6 × 10 −3 to 1.3 × 10 −2 M, and the detection limit is 3.5 × 10 −6 M. The response time of the biosensor is fast (within 10 s), and the life time is over two months. The effects of electroactive interferents, such as ascorbic acid, uric acid, can be significantly reduced by a Nafion film casting on the surface of resulting biosensor.