Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions

• Published in: Food and chemical toxicology 2010-06, Vol.48 (6), p.1537-1542
• Main Authors: Zovko Končić, M., Kremer, D., Gruz, J., Strnad, M., Biševac, G., Kosalec, I., Šamec, D., Piljac-Žegarac, J., Karlović, K.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; anti-infective properties; Antimicrobial activity; Antioxidant activity; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Assaying; Bacteria; Biological and medical sciences; Boraginaceae - chemistry; butylated hydroxyanisole; Chelating; chelating agents; Chromatography, Liquid; Croatia; flavonoids; Flavonoids - analysis; Flowers; Flowers - chemistry; Fungi; iron; leaf extracts; Leaves; Medical sciences; Microbial Sensitivity Tests; Moltkia petraea; Phenol; Phenolic acids; phenols; Phenols - analysis; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Stems - chemistry; stems; Tandem Mass Spectrometry; Total phenols; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2010.03.021

Antioxidant and antimicrobial activities as well as the quantity of phenolic substances (total phenol, flavonoid and phenolic acid contents) were determined in aqueous extracts of leaves, stems and flowers of Moltkia petraea (Tratt.) Griseb. from two mountainous localities (Sveti Jure and Sniježnica) in Croatia. In addition, the profile of phenolic acids was analyzed by UPLC–MS/MS. Antioxidant activities of all extracts in different test systems, namely the DPPH radical scavenging, reducing power assay and chelating activity, increased with extract concentration. Activity of the extracts from Sniježnica in β-carotene-linoleic acid assay did not differ from the activity of standard, BHA. The leaf extracts from Sniježnica demonstrated superior antioxidant activity in most of the assays, while the stem extract from the same locality was the most effective Fe2+ ion chelator. In general, the extracts from Sniježnica were more effective antioxidants than the corresponding extracts from Sveti Jure. The aqueous extracts of M. petraea did not show antimicrobial activity against bacteria and fungi tested in the diffusion and dilution assays.