In vivo genotoxicity and stress defences in three flatfish species exposed to CuSO4

We have used the comet assay to analyse, after 3h, 24h and 6 days, the genotoxic effect in vivo of applying a single intraperitoneal injection of CuSO4, at a concentration of 2mg/kg, to adult specimens of Solea senegalensis, Dicologlossa cuneata and Scophthalmus rhombus. Metals content (Cu, Zn and C...

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Published in:Ecotoxicology and environmental safety 2010-09, Vol.73 (6), p.1279-1285
Main Authors: Chairi, H., Fernández-Diaz, C., Navas, J.I, Manchado, M., Rebordinos, L., Blasco, J.
Format: Article
Language:English
Subjects:
Animals
Antioxidant enzymes
Antioxidants
Biocompatibility
Biomedical materials
Catalase - metabolism
Comet Assay
Copper Sulfate - toxicity
CuSO4
Dicologlossa cuneata
DNA Damage
Enzyme activity
Flatfish
Flatfishes - growth & development
Flatfishes - metabolism
Genotoxicity
Glutathione
Heat-Shock Proteins - metabolism
In vivo testing
In vivo tests
Liver - drug effects
Liver - enzymology
Liver - metabolism
Liver - pathology
Marine
Metallothionein - metabolism
Mutagens - toxicity
Oxidative Stress - drug effects
Reactive Oxygen Species - metabolism
Scophthalmus rhombus
Solea senegalensis
Species Specificity
Stress protein
Superoxide Dismutase - metabolism
Surgical implants
Water Pollutants, Chemical - toxicity
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Summary:We have used the comet assay to analyse, after 3h, 24h and 6 days, the genotoxic effect in vivo of applying a single intraperitoneal injection of CuSO4, at a concentration of 2mg/kg, to adult specimens of Solea senegalensis, Dicologlossa cuneata and Scophthalmus rhombus. Metals content (Cu, Zn and Cd) in liver was also measured. The activity of key stress defences was evaluated by analysing antioxidant enzyme activity (catalase (CAT), superoxide dismutase (SOD), total glutathione peroxidase (t-GPX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH)), metallothionein (MT) and heat shock proteins (HSP70 and HSP60). The results show that CuSO4 intake generates high and cumulative levels of genotoxicity throughout the 6 days in all 3 species. After 6 days, metals content detected in specimens showed significant differences from controls. Inter-species differences were detected in enzyme activity (P
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2010.07.028