Atmospheric pressure-electron capture dissociation of peptides using a modified PhotoSpray ion source

An improved in‐source atmospheric pressure‐electron capture dissociation (AP‐ECD) method is described. Building upon the early example of Laprévote's group, photoelectrons generated within a commercial PhotoSpray atmospheric pressure photoionization source are used to induce ECD of multiply cha...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2010-11, Vol.24 (22), p.3303-3308
Main Authors: Robb, Damon B., Rogalski, Jason C., Kast, Jürgen, Blades, Michael W.
Format: Article
Language:English
Subjects:
Atmospheric Pressure
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Models, Molecular
Peptides - chemistry
Photochemistry - methods
Substance P - chemistry
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Summary:An improved in‐source atmospheric pressure‐electron capture dissociation (AP‐ECD) method is described. Building upon the early example of Laprévote's group, photoelectrons generated within a commercial PhotoSpray atmospheric pressure photoionization source are used to induce ECD of multiply charged peptide ions originating from an upstream heated nebulizer device. To attain high sensitivity, the method makes use of a novel electropneumatic‐heated nebulizer to assist in the creation and transmission of multiply charged ions from sample solutions. Here, we demonstrate that readily interpretable AP‐ECD spectra of infused peptides can be acquired from 100 fmol sample consumed, on a chromatographic time scale, using a conventional quadrupole time‐of‐flight (Q‐ToF) mass spectrometer otherwise incapable of ECD/ETD experiments. Though much work remains to be done to develop and characterize the method, the results indicate that AP‐ECD has the potential to be a practical new tool for the mass spectrometric analysis of peptides and proteins. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.4773