Malignant transformation of cutaneous lesions in renal allograft patients : a role for human papillomavirus ?

Renal allograft recipients are at greatly increased risk of developing squamous cell carcinomas. As these frequently arise adjacent to areas of multiple viral warts, we have investigated a possible role for human papillomavirus in malignant transformation within this population. We established, as p...

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Published in:Cancer research (Chicago, Ill.) Ill.), 1993-11, Vol.53 (21), p.5328-5333
Main Authors: PURDIE, K. J, SEXTON, C. J, PROBY, C. M, GLOVER, M. T, WILLIAMS, A. T, STABLES, J. N, LEIGH, I. M
Format: Article
Language:English
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Summary:Renal allograft recipients are at greatly increased risk of developing squamous cell carcinomas. As these frequently arise adjacent to areas of multiple viral warts, we have investigated a possible role for human papillomavirus in malignant transformation within this population. We established, as primary cultures, keratinocytes from 24 lesions of varying degrees of squamous atypia from 9 patients. Ten of 14 cultures screened for the presence of episomal human papillomavirus DNA were positive using a mixed probe for cutaneous human papillomaviruses, although episomal copy was universally lost with continued passage. Three cultures, 2 of which were derived from malignant tissue and 1 from a benign lesion, were positive when screened with a probe for potentially oncogenic human papillomavirus DNAs 5 or 8. Both positive cultures of malignant origin exhibited extended lifespan and have been briefly characterized by morphology and growth requirements.
ISSN:0008-5472
1538-7445