Soluble CD16 binds peripheral blood mononuclear cells and inhibits pokeweed-mitogen-induced responses

Human neutrophils express two types of low affinity receptors for IgG, Fc gamma RII or CD32 and Fc gamma RIIIB or CD16. Human serum contains soluble CD16 (sCD16), which is produced by proteolysis of neutrophil Fc gamma RIIIB, the cleavage site being located close to the cell surface. In order to ass...

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Bibliographic Details
Published in:Blood 1993-11, Vol.82 (10), p.3081-3090
Main Authors: TEILLAUD, C, GALON, J, ZILBER, M.-T, MAZIERES, N, SPAGNOLI, R, KURRLE, R, FRIDMAN, W. H, SAUTES, C
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Base Sequence
Biological and medical sciences
Cell interactions
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunoglobulin Isotypes - analysis
Immunoglobulins - biosynthesis
Leukocytes, Mononuclear - metabolism
Lymphocyte Activation
Molecular Sequence Data
Pokeweed Mitogens - pharmacology
Receptors, IgG - physiology
Recombinant Proteins - pharmacology
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Summary:Human neutrophils express two types of low affinity receptors for IgG, Fc gamma RII or CD32 and Fc gamma RIIIB or CD16. Human serum contains soluble CD16 (sCD16), which is produced by proteolysis of neutrophil Fc gamma RIIIB, the cleavage site being located close to the cell surface. In order to assess the functional roles of sCD16, we have produced, in eukaryotic cells, a recombinant sCD16 containing the extracellular region of Fc gamma RIIIB. Purified sCD16, of molecular mass of 48 kD, bound human IgG1 and IgG3 but not IgG2, IgG4, or F(ab')2. It inhibited, in a time and dose-dependent fashion, proliferation and IgM and IgG production of human peripheral blood mononuclear cells (PBMC) stimulated by pokeweed mitogen (PWM) in vitro. FACS analysis showed that biotinylated sCD16 bound specifically to a fraction (35%) of PBMC, which corresponds to monocytes and to subsets of B and T lymphocytes. Moreover, sCD16 did not modify the staining of PBMC by FITC-coupled PWM. Thus, the biologic function(s) of sCD16 on PWM-induced responses are exerted through direct and specific interaction(s) with mononuclear blood cells and not with PWM. In conclusion, neutrophils may play a regulatory role on immune responses via the production of soluble forms of CD16 with cell-binding and antiproliferative capacities.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V82.10.3081.3081