The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein

We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was d...

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Bibliographic Details
Published in:Plant molecular biology 1993-10, Vol.23 (2), p.287-295
Main Authors: Prioli, L.M. (Estadual de Campinas Univ. (Brazil). Dept. de Genetica e Evolucao), Huang, J, Levings, C.S. III
Format: Article
Language:English
Subjects:
ADN
ANTIBODIES
ANTICORPS
ANTICUERPOS
Biological and medical sciences
Cell Fractionation
DNA
DNA, Mitochondrial - genetics
Escherichia coli - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genes, Plant
Genes. Genome
GENOMAS
GENOME
GENOMES
IMMUNOPRECIPITATION TESTS
MEMBRANA
MEMBRANE
Membrane Proteins - biosynthesis
Membrane Proteins - genetics
MEMBRANES
MITOCHONDRIA
MITOCHONDRIE
MITOCONDRIA
Molecular and cellular biology
Molecular genetics
Open Reading Frames
Plants - genetics
Protein Biosynthesis
PROTEINAS
PROTEINE
PROTEINS
REACCION DE INMUNOPRECIPITACION
REACTION D'IMMUNOPRECIPITATION
Recombinant Fusion Proteins - biosynthesis
ZEA MAYS
Zea mays - genetics
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Summary:We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00029005