Image and flow cytometry: Companion techniques for adherent and non-adherent cell analysis and sorting

Flow cytometry (FMC) is an analytical and preparative technique whereas image analysis is only applied to cell analysis. Recently, image analysis has been adapted as a preparative method using a new technique: image cytometry for analysis and sorting (ICAS). FCM and ICAS are complementary. Flow cyto...

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Bibliographic Details
Published in:Biology of the cell 1993, Vol.78 (1), p.129-134
Main Author: Métézeau, Philippe
Format: Article
Language:English
Subjects:
Cell Adhesion
Cell Separation - instrumentation
Cell Separation - methods
confocal microscopy
flow cytometry
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fluorescent Antibody Technique
image analysis
image cytometry
Image Processing, Computer-Assisted - instrumentation
Image Processing, Computer-Assisted - methods
pholobleaching
quantitative microscopy
scanning microscopy
Signal Processing, Computer-Assisted
Specimen Handling
Suspensions
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Summary:Flow cytometry (FMC) is an analytical and preparative technique whereas image analysis is only applied to cell analysis. Recently, image analysis has been adapted as a preparative method using a new technique: image cytometry for analysis and sorting (ICAS). FCM and ICAS are complementary. Flow cytometry allows rapid, quantitative and precise study of fluorescence and light scattering in a large number of cells in suspension, while ICAS analyses fewer cells (adherent cells or tissue) on the basis of fluorescence, morphology and size. ICAS can use these criteria to destroy unwanted cells and hence sort selected cells. ICAS can also be used for confocal microscopy and laser surgery.
ISSN:0248-4900
1768-322X
DOI:10.1016/0248-4900(93)90123-V