Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino aci...

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Bibliographic Details
Published in:Plant molecular biology 1993-10, Vol.23 (2), p.423-428
Main Authors: Puupponen-Pimiae, R. (Biotechnical Lab. VTT, Espoo (Finland)), Saloheimo, M, Vasara, T, Ra, R, Gaugecz, J, Kurten, U, Knowles, J.K.C, Keraenen, S, Kauppinen, V
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ARN MENSAJERO
ARN MESSAGER
BETULA PENDULA
Biological and medical sciences
Consensus Sequence
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
EMBRIONES VEGETALES
EMBRYON VEGETAL
EMBRYONIC DEVELOPMENT
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Plant
Genes. Genome
Genome
Growth Substances
Introns - genetics
MESSENGER RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
PLANT EMBRYOS
Plant Proteins - genetics
RNA, Messenger - analysis
SECUENCIA NUCLEICA
Sequence Analysis, DNA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
Trees - embryology
Trees - genetics
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Summary:We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00029018