A → Z transition in the synthetic hexanucleotide (dCdGfl) 3

500 MHz proton NMR and NOE measurements on (dCdGfl) 3 show that at very low ionic strength the hexanucleotide adopts an A-DNA conformation, whereas at high salt concentrations a Z-form is found. At intermediate salt concentrations the two species are in slow exchange on the proton NMR time scale. Th...

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Bibliographic Details
Published in:FEBS letters 1985-03, Vol.182 (2), p.365-369
Main Authors: Fazakerley, G.V., Uesugi, S., Izumi, A., Ikehara, M., Guschlbauer, W.
Format: Article
Language:English
Subjects:
2&#x0301
-Deoxy-2&#x0301
-fluoronucleoside
2́-Deoxy-2́-fluoronucleoside
Biological and medical sciences
CD, circular dichroism
Circular Dichroism
Conformation
Conformational dynamics in molecular biology
dGfl, 2́-deoxy-2́-fluoroguanosine
Fundamental and applied biological sciences. Psychology
Magnetic Resonance Spectroscopy
Molecular biophysics
NMR
NOE
NOE, nuclear Overhauser effect
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Oligonucleotides
Osmolar Concentration
Solutions
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Summary:500 MHz proton NMR and NOE measurements on (dCdGfl) 3 show that at very low ionic strength the hexanucleotide adopts an A-DNA conformation, whereas at high salt concentrations a Z-form is found. At intermediate salt concentrations the two species are in slow exchange on the proton NMR time scale. This transition was also observed by characteristic changes in the CD spectra.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80334-3