Non-histone chromatin protein fractions associated with activë chromatin in embryonic chicken liver

Non-histone chromatin proteins synthesized during chicken embryonic liver development were labeled with [3H]tryptophan and [3H]methionine and characterized by electrophoresis. During embryonic development protein/DNA ratio in chromatin was low (1.30-1.62) but synthesis of non-histone protein was hig...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 1985-01, Vol.65 (2), p.131-141
Main Authors: SIEMIENIAKO, B, RAKOWICZ-SZULCZYNSKA, E. M, HORST, A
Format: Article
Language:English
Subjects:
Age Factors
Animals
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Chick Embryo
Chromatin - isolation & purification
Chromatin - metabolism
Chromosomal Proteins, Non-Histone - metabolism
DNA - metabolism
Electrophoresis, Polyacrylamide Gel
Endodeoxyribonucleases
Fundamental and applied biological sciences. Psychology
Liver - metabolism
Molecular and cellular biology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Non-histone chromatin proteins synthesized during chicken embryonic liver development were labeled with [3H]tryptophan and [3H]methionine and characterized by electrophoresis. During embryonic development protein/DNA ratio in chromatin was low (1.30-1.62) but synthesis of non-histone protein was high. Especially one characteristic fraction K (MW 18 000), tightly bound with DNA was preferentially associated with DNAase II sensitive, active transcribed sequences. In 7-day old and adult chicken synthesis of all non-histone proteins was low, fraction K was absent or synthesized only in small amounts in association with non-active sequences, however protein/DNA ratio in chromatin was high (2.30-2.33).
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00221096