Detection of Human T Lymphotropic Virus Type II/b in Human Immunodeficiency Virus Type 1-Coinfected Persons in Southeastern Italy

A preliminary screening of 511 persons at risk for AIDS living in southeastern Italy disclosed 20 cases of seroreactivity to human T Iymphotropic viruses (HTLV). To verify and type the HTLV infection among these subjects, confirmatory serologic tests, polymerase chain reaction (PCR), and virus cultu...

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Bibliographic Details
Published in:The Journal of infectious diseases 1993-11, Vol.168 (5), p.1273-1277
Main Authors: Calabró, Maria Luisa, Luparello, Monica, Grottola, Antonella, Mistro, Annarosa Del, Fiore, Jose R., Angarano, Gioacchino, Bianchi, L. Chieco
Format: Article
Language:English
Subjects:
AIDS
AIDS/HIV
Antigens
Base Sequence
Biological and medical sciences
Concise Communications
Genes, env
HIV 1
HIV Seropositivity - complications
HTLV II infections
HTLV-II Infections - blood
HTLV-II Infections - complications
HTLV-II Infections - epidemiology
HTLV-II Infections - genetics
Human T lymphotropic virus 1
Human T lymphotropic virus 2
Human T-lymphotropic virus 2 - growth & development
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunoenzyme Techniques
Immunopathology
Italy - epidemiology
Medical sciences
Molecular Sequence Data
Polymerase Chain Reaction
Reactivity
Restriction Mapping
Risk Factors
Serotyping
T lymphocytes
Virus Replication
Viruses
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Description
Summary:A preliminary screening of 511 persons at risk for AIDS living in southeastern Italy disclosed 20 cases of seroreactivity to human T Iymphotropic viruses (HTLV). To verify and type the HTLV infection among these subjects, confirmatory serologic tests, polymerase chain reaction (PCR), and virus culture were done. No evidence of HTLV-I infection was found. HTLV-II infection was confirmed in 8 cases by HTLV-specific, synthetic peptide EIAs and peR on uncultured cells; restriction analysis ofthe peR-amplified env regions revealed the presence of HTL-VII/b strains in all 8 cases. Four sera were non typeable by EIA. The finding of such indeterminate reactivities in a geographic area in which HTLV variants were previously described indicates the need for more extensive surveys among the healthy population. HTLV-II was isolated in 5 cases, and virus isolation was mostly dependent on the presence of an actively replicating human immunodeficiency virus type 1 in culture.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/168.5.1273