High-Pressure Liquid Chromatographic Assay of Theophylline in Dog Feces following Oral Administration of Sustained-Release Products

A solid-phase-extraction reversed-phase HPLC assay is described for the determination of theophylline embedded in dog feces as powder, sustained-release tablets, or capsules. The feces is extracted with 5% isopropyl alcohol in chloroform in the presence of β-hydroxypropyl-theophylline as the interna...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1993-09, Vol.82 (9), p.956-958
Main Authors: Chow, Andrew T., Meek, Patrick D., Jusko, William J.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Biological Availability
Capsules
Chromatography, High Pressure Liquid
Delayed-Action Preparations
Dogs
Feces - chemistry
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Tablets
Theophylline - administration & dosage
Theophylline - analysis
Theophylline - pharmacokinetics
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Summary:A solid-phase-extraction reversed-phase HPLC assay is described for the determination of theophylline embedded in dog feces as powder, sustained-release tablets, or capsules. The feces is extracted with 5% isopropyl alcohol in chloroform in the presence of β-hydroxypropyl-theophylline as the internal standard. Separation and quantitation are achieved with a C18 analytical column. UV absorbance is monitored at 280nm. Recovery of theophylline was >50%. The assay is linear between 10 and 400mg amounts of theophylline in 50g of feces. Inter- and intraday coefficients of variation of the chromatographic assay were
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600820917