Characterization of Envelope and Core Structural Gene Products of HTLV-III with Sera from AIDS Patients

The envelope (env) and structural (gag) gene products of human T-cell leukemia (lymphotropic) virus type III were identified by immunoaffinity chromatography, immunoprecipitation, and two-dimensional oligopeptide mapping methods. The env gene specifies a glycosylated polypeptide with a molecular wei...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1985-05, Vol.228 (4699), p.593-595
Main Authors: Robey, W. Gerard, Safai, Bijan, Oroszlan, Stephen, Arthur, Larry O., Gonda, Matthew A., Gallo, Robert C., Fischinger, Peter J.
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - microbiology
affinity chromatography
AIDS
AIDS (Disease)
AIDS research
AIDS/HIV
Animals
Antibodies
Antibodies, Viral - immunology
Biological and medical sciences
Cancer
Cancer research
Chromatography, Affinity
Deltaretrovirus - metabolism
Fundamental and applied biological sciences. Psychology
gene products
Genes, Viral
Glycoproteins
HIV
Humans
Immunology, Experimental
Immunoprecipitation
Microbiology
Molecular Weight
Molecules
Oncology, Experimental
Pan troglodytes
peptide mapping
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Sarcoma, Kaposi - microbiology
T cell leukemia virus III
Viral Envelope Proteins - isolation & purification
Viral Proteins - isolation & purification
Virions
Virology
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The envelope (env) and structural (gag) gene products of human T-cell leukemia (lymphotropic) virus type III were identified by immunoaffinity chromatography, immunoprecipitation, and two-dimensional oligopeptide mapping methods. The env gene specifies a glycosylated polypeptide with a molecular weight of 160,000 (gp 160) that is processed to gp 120 and smaller gene products. The gag gene specifies two polypeptides of 70,000 and 55,000 molecular weight (p70 and p55), both of which contain p24, the major structural protein of the mature virion. The techniques in this study can be used to define the extent of variability of the env gene product among different virus isolates and may identify the nature and patterns of the humoral immune response that lead to an immunologically protected state.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2984774