Amphotericin B enhances efficiency of DNA-mediated gene transfer in mammalian cells

Using plasmids containing the genes for thymidine kinase (tk) and neomycin resistance (neo), we have shown that DNA-mediated genotypic transformation of L and Chinese hamster ovary (CHO) cells is increased several-fold by the presence of the sterol-binding polyene antibiotic, amphotericin. Transform...

Full description

Saved in:
Bibliographic Details
Published in:Somatic cell and molecular genetics 1985-03, Vol.11 (2), p.109-115
Main Authors: HIDAKA, K, AN, G, IP, P, KUWANA, M, SIMINOVITCH, L
Format: Article
Language:English
Subjects:
Amphotericin B - pharmacology
Animals
beta-Galactosidase - genetics
Biological and medical sciences
Biotechnology
Cell Line
Cricetinae
DNA - genetics
DNA, Recombinant
Female
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
L Cells (Cell Line)
Methods. Procedures. Technologies
Mice
Neomycin - pharmacology
Ovary
Plasmids
R Factors
Thymidine Kinase - genetics
Transfection - drug effects
Transformation, Genetic - drug effects
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using plasmids containing the genes for thymidine kinase (tk) and neomycin resistance (neo), we have shown that DNA-mediated genotypic transformation of L and Chinese hamster ovary (CHO) cells is increased several-fold by the presence of the sterol-binding polyene antibiotic, amphotericin. Transformation into the same host cells, using genomic DNA, was also enhanced by amphotericin. Phenotypic expression of beta-galactosidase activity of a plasmid containing the gene for the enzyme was also markedly elevated when the antibiotic was added at transfection. Other sterol-binding polyene antibiotics also showed activity in these DNA-mediated gene transfer assays.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01534699