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Cloning and Nucleotide Sequence Determination of the Clostridium pasteurianum Ferredoxin Gene
We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain >...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1985-03, Vol.82 (6), p.1653-1657 |
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| Main Authors: | , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c516t-e7982acf18f331bc50e3f65fbef4a1844e2d04282faec59957a977e1118b87213 |
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| container_end_page | 1657 |
| container_issue | 6 |
| container_start_page | 1653 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 82 |
| creator | Graves, Mary C. Mullenbach, Guy T. Rabinowitz, Jesse C. |
| description | We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain >6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobasepair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence. |
| doi_str_mv | 10.1073/pnas.82.6.1653 |
| format | article |
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Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain >6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobasepair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.6.1653</identifier><identifier>PMID: 3856844</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; Clostridium - genetics ; Clostridium pasteurianum ; Codon - genetics ; DNA ; DNA, Bacterial - genetics ; Ferredoxins - genetics ; Fundamental and applied biological sciences. Psychology ; Gels ; Genes, Bacterial ; Genetic engineering ; Genetic technics ; Genomics ; Messenger RNA ; Methods. Procedures. Technologies ; Molecular cloning ; Nucleotide sequences ; Nucleotides ; Oligonucleotide probes ; Open reading frames ; Operon ; Plasmids ; Promoter regions ; Protein Biosynthesis ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (6), p.1653-1657</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-e7982acf18f331bc50e3f65fbef4a1844e2d04282faec59957a977e1118b87213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25476$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25476$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791,58236,58469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9281321$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3856844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graves, Mary C.</creatorcontrib><creatorcontrib>Mullenbach, Guy T.</creatorcontrib><creatorcontrib>Rabinowitz, Jesse C.</creatorcontrib><title>Cloning and Nucleotide Sequence Determination of the Clostridium pasteurianum Ferredoxin Gene</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain >6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobasepair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Clostridium - genetics</subject><subject>Clostridium pasteurianum</subject><subject>Codon - genetics</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Ferredoxins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genes, Bacterial</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genomics</subject><subject>Messenger RNA</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Nucleotide sequences</subject><subject>Nucleotides</subject><subject>Oligonucleotide probes</subject><subject>Open reading frames</subject><subject>Operon</subject><subject>Plasmids</subject><subject>Promoter regions</subject><subject>Protein Biosynthesis</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqFkc1rFDEYxoModa1ePQjCHMTbjPmYZJKDh7LaKhQ9qEcJ2cybNmU2WZOM1P_eLLsOKwieQnh-z_v1IPSc4I7ggb3ZBZM7STvREcHZA7QiWJFW9Ao_RCuM6dDKnvaP0ZOc7zDGikt8hs6Y5EL2_Qp9X08x-HDTmDA2n2Y7QSx-hOYL_JghWGjeQYG09cEUH0MTXVNuoammXJIf_bxtdiYXmJM3oX4uISUY470PzRUEeIoeOTNleHZ8z9G3y_df1x_a689XH9cX163lRJQWBiWpsY5IxxjZWI6BOcHdBlxvSJ0T6Ih7KqkzYLlSfDBqGIAQIjdyoISdo7eHurt5s4XRQijJTHqX_NakXzoar_9Wgr_VN_GnZmpgDFf_66M_xbp3Lnrrs4VpMgHinPUgsKinJP8FSU85lUpVsDuANsWcE7hlGIL1Pji9D05LqoXeB1cNL09XWPBjUlV_ddRNtmZyyQTr84IpKgmjpwPuy_9RlzbazdNU4L6c9PsnWPUXB_0ul5gWgPJ-EOw3ozXDjQ</recordid><startdate>19850301</startdate><enddate>19850301</enddate><creator>Graves, Mary C.</creator><creator>Mullenbach, Guy T.</creator><creator>Rabinowitz, Jesse C.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850301</creationdate><title>Cloning and Nucleotide Sequence Determination of the Clostridium pasteurianum Ferredoxin Gene</title><author>Graves, Mary C. ; Mullenbach, Guy T. ; Rabinowitz, Jesse C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-e7982acf18f331bc50e3f65fbef4a1844e2d04282faec59957a977e1118b87213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Clostridium - genetics</topic><topic>Clostridium pasteurianum</topic><topic>Codon - genetics</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Ferredoxins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genes, Bacterial</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genomics</topic><topic>Messenger RNA</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Nucleotide sequences</topic><topic>Nucleotides</topic><topic>Oligonucleotide probes</topic><topic>Open reading frames</topic><topic>Operon</topic><topic>Plasmids</topic><topic>Promoter regions</topic><topic>Protein Biosynthesis</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graves, Mary C.</creatorcontrib><creatorcontrib>Mullenbach, Guy T.</creatorcontrib><creatorcontrib>Rabinowitz, Jesse C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graves, Mary C.</au><au>Mullenbach, Guy T.</au><au>Rabinowitz, Jesse C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Nucleotide Sequence Determination of the Clostridium pasteurianum Ferredoxin Gene</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-03-01</date><risdate>1985</risdate><volume>82</volume><issue>6</issue><spage>1653</spage><epage>1657</epage><pages>1653-1657</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain >6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobasepair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3856844</pmid><doi>10.1073/pnas.82.6.1653</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-03, Vol.82 (6), p.1653-1657 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76060271 |
| source | PubMed; JSTOR Archival Journals |
| subjects | Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Clostridium - genetics Clostridium pasteurianum Codon - genetics DNA DNA, Bacterial - genetics Ferredoxins - genetics Fundamental and applied biological sciences. Psychology Gels Genes, Bacterial Genetic engineering Genetic technics Genomics Messenger RNA Methods. Procedures. Technologies Molecular cloning Nucleotide sequences Nucleotides Oligonucleotide probes Open reading frames Operon Plasmids Promoter regions Protein Biosynthesis Transcription, Genetic |
| title | Cloning and Nucleotide Sequence Determination of the Clostridium pasteurianum Ferredoxin Gene |
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