Assay of serum cholinesterase with succinylcholine and propionylthiocholine as substrates

Serum cholinesterase (E.C. 3.1.1.8) was assayed with succinylcholine as a substrate. The reaction was coupled with choline oxidase and peroxidase in the presence of 4-aminoantipyrine and phenol to produce a red quinone dye that was measured spectrophotometrically. The method requires 25 microliter o...

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Bibliographic Details
Published in:Anesthesiology (Philadelphia) 1985-04, Vol.62 (4), p.509-512
Main Authors: WAKID, N. W, RAMZI TUBBEH, ANIS BARAKA
Format: Article
Language:English
Subjects:
Alcohol Oxidoreductases
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Choline - analogs & derivatives
Cholinesterases - blood
Cholinesterases - genetics
Chromogenic Compounds - analysis
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Genotype
Horseradish Peroxidase
Humans
Hydrogen-Ion Concentration
Hydrolases
Hydrolysis
Quinones - analysis
Reference Values
Spectrophotometry
Succinylcholine
Thiocholine - analogs & derivatives
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Summary:Serum cholinesterase (E.C. 3.1.1.8) was assayed with succinylcholine as a substrate. The reaction was coupled with choline oxidase and peroxidase in the presence of 4-aminoantipyrine and phenol to produce a red quinone dye that was measured spectrophotometrically. The method requires 25 microliter of sample in a total volume of 1.0 ml. The mean activity for 35 adults of the usual genotype was 74.4 +/- 28 U/l (range 24-125 U/l). Succinylcholine-sensitive individuals had activities below 18 U/l. The same serum samples also were assayed with propionylthiocholine as a substrate. Activities with the two substrates showed a coefficient of correlation of 0.980 (n = 68). However, the method using propionylthiocholine showed more overlap between the activities of succinylcholine-sensitive and insensitive individuals. Assay with succinylcholine thus may offer a more effective method of screening for sensitive individuals, since some escape detection by conventional genotyping with dibucaine and fluoride.
ISSN:0003-3022
1528-1175
DOI:10.1097/00000542-198504000-00023