Murine cytotoxic T cell response specific for human cytomegalovirus glycoprotein B (gB) induced by adenovirus and vaccinia virus recombinants expressing gB

• Published in: Journal of general virology 1993-11, Vol.74 (11), p.2507-2512
• Main Authors: Berencsi, Klara, Rando, Robert F, deTaisne, Charles, Paoletti, Enzo, Plotkin, Stanley A, Gonczol, Eva
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenoviridae - immunology; adenovirus; Animals; Biological and medical sciences; Biotechnology; Cytomegalovirus - immunology; Fundamental and applied biological sciences. Psychology; Humans; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Microbiology; Models, Biological; Recombinant Fusion Proteins - immunology; T-Lymphocytes, Cytotoxic - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology; vaccinia virus; Vaccinia virus - genetics; Vaccinia virus - immunology; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Vaccines - immunology; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-74-11-2507

The 1 Wistar Institute of Anatomy and Biology, 3601 Spruce Street, Philadelphia, Pennsylvania 19104-4268, U.S.A. 2 National Institute of Dental Research, 9000 Rockville Pike, Bethesda, Maryland 20892, U.S.A. 3 Institut Pasteur, Merieux, 1541 Avenue Marcel-Merieux, 69280 Marcy-l'Etoile, France and 4 Virogenetics Corporation, Rensselaer Technology Park, 465 Jordan Road, Troy, New York 12180, U.S.A. A murine model of the cytotoxic T lymphocyte (CTL) response to glycoprotein B (gB) of human cytomegalovirus (HCMV) was developed based on the use of adenovirus (Ad) and vaccinia virus (Vac) recombinants expressing gB. Mice of different major histocompatibility haplotypes [CBA (H-2 k ), BALB/k (H-2 k ) and BALB/c (H-2 d )] infected with the Ad-gB recombinant developed an Ad-specific CTL response. However, only the H-2 k mice developed a significant HCMV gB-specific CTL response, as indicated by the major histocompatibility complex class I-restricted lysis of Vac strain Copenhagen (VacC)-gB recombinant-infected target cells by H-2 k mouse immune spleen cells. The VacC-gB recombinant elicited only a weak gB-specific CTL response in these mice, indicating that the observed gB-specific CTL response in mice is dependent on the expression vector used for immunization. The gB-specific cytotoxicity observed in H-2 k mice was mediated by the CD8 lymphocyte subset. Present address: Triplex Pharmaceutical Corporation, 9391 Grogen's Mill Road, The Woodlands, Texas 77380, U.S.A. Received 1 June 1993; accepted 20 July 1993.