Melanin biosynthesis patterns following hormonal stimulation

The effect of melanocyte-stimulating hormone (MSH) on the differentiation of mammalian melanocytes has been widely studied since the early 1950s. There have been many reports about the stimulatory effect of MSH on melanin production and specifically on the activity of tyrosinase, the critical enzyme...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-12, Vol.268 (34), p.25650-25655
Main Authors: Aroca, P, Urabe, K, Kobayashi, T, Tsukamoto, K, Hearing, V J
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Aromatic and heterocyclic compounds
Biological and medical sciences
Blotting, Northern
Fundamental and applied biological sciences. Psychology
Heterocyclic compounds, pigments
Intramolecular Oxidoreductases
Isomerases - metabolism
Kinetics
Melanins - biosynthesis
Melanocyte-Stimulating Hormones - pharmacology
Melanoma, Experimental - metabolism
Melanoma, Experimental - pathology
Mice
Models, Biological
Monophenol Monooxygenase - metabolism
Other biological molecules
Phenotype
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Tumor Cells, Cultured
Tyrosine 3-Monooxygenase - metabolism
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Summary:The effect of melanocyte-stimulating hormone (MSH) on the differentiation of mammalian melanocytes has been widely studied since the early 1950s. There have been many reports about the stimulatory effect of MSH on melanin production and specifically on the activity of tyrosinase, the critical enzyme in the melanogenic pathway. However, few and variable results have been obtained concerning the effect of this hormone on the regulation of DOPAchrome tautomerase (TRP2), another melanogenic enzyme which functions later in the melanogenic pathway, or on other melanogenic activities, such as TRP1. In this study, we show that the MSH-induced stimulation of tyrosinase is accompanied by no significant change in the synthesis or catalytic activities of other melanogenic enzymes such as TRP1 or TRP2. This in turn elicits a dramatic increase in melanin production accompanied by a significant decrease in the incorporation of carboxylated precursors into that melanin biopolymer, although the biological implication of that is still unclear.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)74439-1