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CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection

CD69, an ‘activation marker’ that is rapidly induced on mature T cells after stimulation through the T cell antigen receptor (TCR) was found to be expressed on ∼10% of normal thymocytes. All of these CD69+ thymocytes express αβ TCR, and they include both TCRlowCD4+CD8+ and TCRhighCD4+CD8− or CD4−CD8...

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Published in:International immunology 1993-09, Vol.5 (9), p.1139-1150
Main Authors: Yamashita, Iwao, Nagata, Taeko, Tada, Tomio, Nakayama, Toshinori
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Nagata, Taeko
Tada, Tomio
Nakayama, Toshinori
description CD69, an ‘activation marker’ that is rapidly induced on mature T cells after stimulation through the T cell antigen receptor (TCR) was found to be expressed on ∼10% of normal thymocytes. All of these CD69+ thymocytes express αβ TCR, and they include both TCRlowCD4+CD8+ and TCRhighCD4+CD8− or CD4−CD8+ thymocytes. The CD69+ cells can be further segregated into heat-stable antigen (HSA)+TCR*HSA−TCRhigh and HSA+TCRhigh thymocyte populations. None of CD69+ cells express the mature T cell marker Qa-2. Thus CD69+ cells present in vivo appear phenotyplcally to represent transitional cell populations between immature TCRlowHSA+Qa-2− double-positive cells and mature TCRhighHSA−QA-2+ single-positive cells. In addition, TCR engagement by MHC molecules is required for CD69 expression in the thymus. Taken together, the CD69 + thymocytes appear to represent the cells auditioning in positive selection process or they are the cells that have been positively selected recently. Analysis of a TCR transgenic mouse model revealed an increased number of CD69+ thymocytes in a positively selecting thymus, whereas no CD69+ transgenic TCR+ thymocytes were observed in the non-selecting thymus. Based on the results of this study, we suggest that the surface expression of CD69 serves as a useful marker to identify and trace those thymocytes that are engaged in the TCR-mediated positive selection process in the thymus.
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Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Immunophenotyping</subject><subject>Lectins, C-Type</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred CBA</subject><subject>positive selection</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - immunology</subject><subject>Spleen - immunology</subject><subject>T cell antigen receptor</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>Thymus Gland - immunology</subject><issn>0953-8178</issn><issn>1460-2377</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkc1v1DAQxS0EKkvhzAnJB8Qtu2M7ieMjLB-ttBWXIqFeLMcZdw35wk5K984fjqNd7bWnkeb93tPYj5C3DNYMlNj4fvJdtynWas2YUM_IiuUlZFxI-ZysQBUiq5isXpJXMf4CAMGVuCAXUgFnAlbk3_ZzqajFtqVxDs5YpPg4BozRDz31DaZ85zHSBh-wHUbf39Npf-gGe5jS9u_e2z01c-OnhXdDoLfHNJOM99jTgBbHaQhZh403EzZ0HGKiH5BGbNEuvtfkhTNtxDeneUl-fP1yu73Kdt-_XW8_7jIrFEyZrY3KnbBQG15zBXWphKwkr3hhSsmsE-BKSE-ouC14VTsoGqk4lMjqykomLsmHY-4Yhj8zxkl3Pi7Xmh6HOWpZQpVzJZ8EWSnzHIAncHMEbRhiDOj0GHxnwkEz0EtB-liQLrTSS0HJ8e4UPdfpS878qZGkvz_pJlrTumB66-MZE7KQEhYsO2I-Tvh4lk34rUuZIH31807fiDt582mXLhH_Ad8Jq5c</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Yamashita, Iwao</creator><creator>Nagata, Taeko</creator><creator>Tada, Tomio</creator><creator>Nakayama, Toshinori</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection</title><author>Yamashita, Iwao ; Nagata, Taeko ; Tada, Tomio ; Nakayama, Toshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-cba94f3c0ba2b290b6937872825a671cf30f60ace82c528bf05d79206e1b8c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>activation marker</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens, CD - immunology</topic><topic>Antigens, Differentiation, T-Lymphocyte - immunology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>CD4+CD8+ thymocytes</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD69</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Immunophenotyping</topic><topic>Lectins, C-Type</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred CBA</topic><topic>positive selection</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - immunology</topic><topic>Spleen - immunology</topic><topic>T cell antigen receptor</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>Thymus Gland - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamashita, Iwao</creatorcontrib><creatorcontrib>Nagata, Taeko</creatorcontrib><creatorcontrib>Tada, Tomio</creatorcontrib><creatorcontrib>Nakayama, Toshinori</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamashita, Iwao</au><au>Nagata, Taeko</au><au>Tada, Tomio</au><au>Nakayama, Toshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection</atitle><jtitle>International immunology</jtitle><addtitle>Int Immunol</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>5</volume><issue>9</issue><spage>1139</spage><epage>1150</epage><pages>1139-1150</pages><issn>0953-8178</issn><eissn>1460-2377</eissn><abstract>CD69, an ‘activation marker’ that is rapidly induced on mature T cells after stimulation through the T cell antigen receptor (TCR) was found to be expressed on ∼10% of normal thymocytes. 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ispartof International immunology, 1993-09, Vol.5 (9), p.1139-1150
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1460-2377
language eng
recordid cdi_proquest_miscellaneous_76084297
source Oxford University Press Archive
subjects activation marker
Animals
Antibodies, Monoclonal
Antigens, CD - immunology
Antigens, Differentiation, T-Lymphocyte - immunology
Biological and medical sciences
Biomarkers
CD4+CD8+ thymocytes
CD4-Positive T-Lymphocytes - immunology
CD69
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Immunophenotyping
Lectins, C-Type
Lymphocyte Activation - immunology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
positive selection
Receptors, Antigen, T-Cell, alpha-beta - immunology
Spleen - immunology
T cell antigen receptor
T-Lymphocytes, Regulatory - immunology
Thymus Gland - immunology
title CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection
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