Impairment of nucleoid segregation and cell division at high osmolarity in a strain of Escherichia coli overproducing the chaperone DnaK

Escherichia coli strain WM1390, which overproduces 20‐fold the chaperone protein DnaK, was able to grow exponentially without apparent abnormality in 300 mosM medium. In contrast, it showed both aberrant nucleoid segregation and strong inhibition of septation when shifted to high osmolarity. These i...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1993-10, Vol.113 (1), p.93-99
Main Authors: Meury, Jean, Bahloul, Abdelkader, Kohiyama, Masamichi
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Bacteriology
Betaine - pharmacology
Biological and medical sciences
Cell Division
Cell Nucleus - ultrastructure
Chaperone
DnaK
Echerichia coli
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - metabolism
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Growth, nutrition, cell differenciation
Heat-Shock Proteins - metabolism
HSP70 Heat-Shock Proteins
Microbiology
Osmolar Concentration
Osmotic‐upshock
Species Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Escherichia coli strain WM1390, which overproduces 20‐fold the chaperone protein DnaK, was able to grow exponentially without apparent abnormality in 300 mosM medium. In contrast, it showed both aberrant nucleoid segregation and strong inhibition of septation when shifted to high osmolarity. These impairments could not be accounted for by a bacteriocidal effect of the high DnaK content. Rather, the DnaK content appeared to promote faster growth than that of the parent C600, at least at high osmolarity in the presence of the osmoprotectant glycine betaine.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06494.x