Ebola protein analyses for the determination of genetic organization

Amino-acid sequencing of the purified major nucleoprotein (NP), VP35 and VP40 from purified Ebola virus proved that they are the protein products of the first three genes, and that the open reading frame (ORF) of the NP begins at nucleotide 470. Because of the many unusual features of the ORFs of Eb...

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Bibliographic Details
Published in:Archives of virology 1993-01, Vol.133 (3-4), p.423-436
Main Authors: ELLIOTT, L. H, SANCHEZ, A, HOLLOWAY, B. P, KILEY, M. P, MCCORMICK, J. B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Blotting, Western
Cercopithecus aethiops
Chromatography, High Pressure Liquid
Clone Cells
Ebola virus
Ebolavirus - chemistry
Ebolavirus - genetics
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetics
Guinea Pigs
Microbiology
Molecular Sequence Data
Nucleoproteins - chemistry
Nucleoproteins - genetics
Nucleoproteins - isolation & purification
Open Reading Frames - genetics
Protein Biosynthesis
Radioimmunoprecipitation Assay
Vero Cells
Viral Core Proteins - chemistry
Viral Core Proteins - genetics
Viral Core Proteins - isolation & purification
Viral Matrix Proteins
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - isolation & purification
Viral Regulatory and Accessory Proteins
Virology
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Summary:Amino-acid sequencing of the purified major nucleoprotein (NP), VP35 and VP40 from purified Ebola virus proved that they are the protein products of the first three genes, and that the open reading frame (ORF) of the NP begins at nucleotide 470. Because of the many unusual features of the ORFs of Ebola virus, we thought that our conclusions should be substantiated. Comparisons of in vitro-translation products to purified viral proteins were used to demonstrate conclusively that the NP, VP35 and VP40 were the protein products of genes one, two, and three, respectively. Studies using antibodies to synthetic peptides matching the N- and C-termini of the deduced sequences from these genes confirmed these conclusions and that the ORF for the NP begins at nucleotide 470. Subsequent studies confirmed that VP30 is encoded by the fifth gene.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01313780