Monoclonal antibodies to Mycoplasma gallisepticum membrane proteins

Monoclonal antibodies (MAbs) were prepared to study the immunogenesis of Mycoplasma gallisepticum. Balb/c mice were immunized with M. gallisepticum immunostimulating complexes and the supernatant of heterokaryotes screened with M. gallisepticum and closely related M. synoviae as antigens in indirect...

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Bibliographic Details
Published in:Avian diseases 1993-07, Vol.37 (3), p.689-696
Main Authors: Czifra, G, Tuboly, T, Sundquist, B.G, Stipkovits, L
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Bacterial
Antibodies, Monoclonal
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIGENE
ANTIGENOS
Antigens
Bacterial Proteins - chemistry
Bacterial Proteins - immunology
Bacterial Proteins - isolation & purification
Cellulose nitrate
Chickens
Enzyme linked immunosorbent assay
Immunization
Immuno stimulating complex
Immunoblotting
IMMUNOSTIMULATION
INMUNOESTIMULACION
ISCOMs - immunology
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Membrane Proteins - chemistry
Membrane Proteins - immunology
Membrane Proteins - isolation & purification
Mice
Mice, Inbred BALB C
Molecular Weight
Mycoplasma
Mycoplasma - immunology
MYCOPLASMA GALLISEPTICUM
RATON
SOURIS
Species Specificity
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Description
Summary:Monoclonal antibodies (MAbs) were prepared to study the immunogenesis of Mycoplasma gallisepticum. Balb/c mice were immunized with M. gallisepticum immunostimulating complexes and the supernatant of heterokaryotes screened with M. gallisepticum and closely related M. synoviae as antigens in indirect enzyme-linked immunosorbent assay. All selected MAbs proved to be M. gallisepticum species-specific when they were tested against 10 different avian Mycoplasma species. After immunoblotting analysis, five polypeptides were identified with estimated molecular weights of 110,000, 66,000, 64,000, 56,000, and 50,000. Cell membrane localization of the recognized polypeptides was studied by immunoelectron microscopy. None of the MAbs inhibited the hemagglutinating activity of freshly prepared M. gallisepticum. However, one MAb (B3) specific for p56 agglutinated the stained M. gallisepticum antigen in the slide agglutination test. Results seemed to correlate with published information on the protein composition and agglutinating activity of Mycoplasma gallisepticum
ISSN:0005-2086
1938-4351
DOI:10.2307/1592016