Deuterium NMR spectroscopy of biofluids for the identification of drug metabolites: Application to N, N-dimethylformamide

The metabolism of N, N-dimethylformamide in the Sprague—Dawley rat has been reinvestigated using NMR spectroscopy of urine. In particular, through the use of N, N-dimethylformamide-d 7 (DMF-d 7) and 2H-NMR spectroscopy, the principal metabolites of this compound have been confirmed in a direct manne...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1993-08, Vol.11 (8), p.687-692
Main Authors: Farrant, R.Duncan, Salman, Salman R., Lindon, John C., Cupid, Belinda C., Nicholson, Jeremy K.
Format: Article
Language:English
Subjects:
2H-NMR
2H 13C correlation
Analysis
Animals
Biological and medical sciences
Deuterium
Dimethylformamide - metabolism
DMF
General pharmacology
Injections, Intraperitoneal
Magnetic Resonance Spectroscopy
Medical sciences
metabolism
Pharmacology. Drug treatments
Rats
Rats, Sprague-Dawley
urine
Urine - chemistry
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Summary:The metabolism of N, N-dimethylformamide in the Sprague—Dawley rat has been reinvestigated using NMR spectroscopy of urine. In particular, through the use of N, N-dimethylformamide-d 7 (DMF-d 7) and 2H-NMR spectroscopy, the principal metabolites of this compound have been confirmed in a direct manner. The use of inverse-detected two-dimensional 2H 13C correlation with 13C decoupling aided metabolite identification through the provision of 13C chemical shifts.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(93)80175-Z