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Development and application of a polymerase chain reaction assay for Mycoplasma synoviae

Mycoplasma synoviae (MS) species-specific primers selected from the 16S rRNA sequence were evaluated by polymerase chain reaction (PCR). The MS primers were MS-1 (5'-GAAGCAAAATAGTGATATCA-3') and MS-2 5'-GTCGTCTCCGAAGTRAACAA-3'). Analysis of cultures of avian mycoplasmas using the...

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Bibliographic Details
Published in:Avian diseases 1993-07, Vol.37 (3), p.829-834
Main Authors: Lauerman, L.H, Hoerr, F.J, Sharpton, A.R, Shah, S.M, Van Santen, V.L
Format: Article
Language:English
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Summary:Mycoplasma synoviae (MS) species-specific primers selected from the 16S rRNA sequence were evaluated by polymerase chain reaction (PCR). The MS primers were MS-1 (5'-GAAGCAAAATAGTGATATCA-3') and MS-2 5'-GTCGTCTCCGAAGTRAACAA-3'). Analysis of cultures of avian mycoplasmas using the MS PCR indicated 100% specificity and sensitivity: 55 individual isolates of MS tested. PCR-positive, and 44 isolates of eight other species of avian mycoplasmas tested PCR-negative. The MS PCR will detect 100 colony-forming units of MS. Analysis of 122 flock data sets indicated a sensitivity for the MS PCR test of 82% and a specificity of 100% as determined by comparison with culture, serology (serum plate test, hemagglutination-inhibition, enzyme-linked immunosorbent assay), epizootiology, and history
ISSN:0005-2086
1938-4351
DOI:10.2307/1592037