A carrageenan hydrogel stabilized colloidal gold multi-enzyme biosensor electrode utilizing immobilized horseradish peroxidase and cholesterol oxidase/cholesterol esterase to detect cholesterol in serum and whole blood

The preparation of two immobilized enzyme electrodes is described. One electrode contains horseradish peroxidase adsorbed to colloidal gold and deposited on a glassy carbon electrode along with cholesterol oxidase entrapped in a carrageenan hydrogel. The second electrode also includes cholesterol es...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1993, Vol.8 (6), p.331-337
Main Authors: Crumbliss, A.L., Stonehuerner, J.G., Henkens, R.W., Zhao, J., O'Daly, J.P.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques
biosensor
Biosensors
Biotechnology
Carrageenan
cholesterol
Cholesterol - blood
Cholesterol Oxidase
colloidal gold
electrode
Electrodes
Enzymes, Immobilized
Fundamental and applied biological sciences. Psychology
Gold Colloid
Horseradish Peroxidase
Humans
hydrogel
Methods. Procedures. Technologies
Sterol Esterase
Various methods and equipments
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Description
Summary:The preparation of two immobilized enzyme electrodes is described. One electrode contains horseradish peroxidase adsorbed to colloidal gold and deposited on a glassy carbon electrode along with cholesterol oxidase entrapped in a carrageenan hydrogel. The second electrode also includes cholesterol esterase entrapped in the carrageenan. The incorporation of ferrocene or ferrocenecarboxylic acid mediator is brought about by either evaporation on the glassy carbon electrode or, in the latter case, entrapment in the carrageenan hydrogel. Amperometric signal generation results from the HRP catalyzed turnover of H 2O 2, a secondary product of the cholesterol oxidase catalyzed oxidation of cholesterol. Use of these enzyme electrodes makes cholesterol detection possible in human serum, low density lipoprotein, and whole blood.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(93)85014-F