Synergistic effects of ascorbic acid and thiazolidinedione on secretion of high molecular weight adiponectin from human adipocytes

Aim: To test the hypothesis that ascorbic acid (AA) and thiazolidinedione (TZD) would have additive effects on HMW adiponectin secretion by virtue of different modes of action. Methods: We determined the effects of supplementation of AA and/or TZD on expression and secretion of total and HMW adipone...

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Bibliographic Details
Published in:Diabetes, obesity & metabolism obesity & metabolism, 2010-12, Vol.12 (12), p.1084-1089
Main Authors: Rose, F.J, Webster, J, Barry, J.B, Phillips, L.K, Richards, A.A, Whitehead, J.P
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - drug effects
Adipocytes - secretion
adipokine
Adiponectin
Adiponectin - secretion
Ascorbic acid
Ascorbic Acid - pharmacology
Clinical trials
Diabetes
Drug Synergism
Humans
Inflammation
Mass Spectrometry
Molecular Weight
obesity
post-translational modification
Supplements
Thiazolidinediones - pharmacology
Tumor necrosis factor-α
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Summary:Aim: To test the hypothesis that ascorbic acid (AA) and thiazolidinedione (TZD) would have additive effects on HMW adiponectin secretion by virtue of different modes of action. Methods: We determined the effects of supplementation of AA and/or TZD on expression and secretion of total and HMW adiponectin from human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes in the absence or presence of the proinflammatory cytokine TNFα. Results: AA supplementation significantly increased secretion of HMW adiponectin (1.7‐fold) without altering adiponectin expression or total adiponectin secretion. TZD significantly increased expression (3‐fold) and secretion of total (1.4‐fold) but not HMW adiponectin. Combined supplementation resulted in a significant increase in expression (3‐fold) and secretion of total (1.8‐fold) and HMW (5‐fold) adiponectin. Similar results were seen in cells co‐treated with TNFα. Conclusions: These data show that AA and TZD have synergistic rather than simple additive effects on secretion of HMW adiponectin from human adipocytes and raise the possibility that differences in AA levels may contribute to the variability in adiponectin multimer profiles and efficacy of TZD in humans. Our results also provide a rationale for longitudinal clinical trials investigating the effects of AA supplementation with or without TZD on adiponectin and metabolic profiles.
ISSN:1462-8902
1463-1326
DOI:10.1111/j.1463-1326.2010.01297.x