Internal ribosome entry site of encephalomyocarditis virus RNA is unable to direct translation in Saccharomyces cerevisiae

To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E. coli-yeast shuttle vectors in which the EMCV 5' non-coding region was fused to the reporter...

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Bibliographic Details
Published in:FEBS letters 1993-12, Vol.335 (2), p.273-276
Main Authors: EVSTAFIEVA, A. G, BELETSKY, A. V, BOROVJAGIN, A. V, BOGDANOV, A. A
Format: Article
Language:English
Subjects:
Base Sequence
DNA Primers
encephalomyocarditis virus
Encephalomyocarditis virus - genetics
Escherichia coli
HeLa Cells
Humans
Molecular Sequence Data
Protein Biosynthesis
Protein Precursors - genetics
Ribosomes - microbiology
RNA, Messenger - genetics
RNA, Viral - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Thymosin - analogs & derivatives
Thymosin - genetics
Virus Integration - genetics
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Summary:To evaluate the potential of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) to promote efficient expression of foreign genes in the yeast, S. cerevisiae, we have constructed E. coli-yeast shuttle vectors in which the EMCV 5' non-coding region was fused to the reporter gene, human prothymosin alpha. Efficiency of translation of corresponding RNA transcripts in mammalian cell-free systems was highly dependent on the sequence context and/or position of the initiation codon. No translation of these IRES-dependent mRNAs occurred in S. cerevisiae.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80745-G