Topography of RNA in the ribosome: location of the 5 S RNA residues A39 and U40 on the central protuberance of the 50 S subunit

The internal site of 5 S RNA comprising residues A39 and U40 has been localized on the E.coli 50 S ribosomal subunit by immune electron microscopy. It has been found to be located on the interface side of the central protuberance at the position distinctly apart but very close to the position of the...

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Bibliographic Details
Published in:FEBS letters 1985-06, Vol.185 (1), p.57-62
Main Authors: Evstafieva, A.G., Shatsky, I.N., Bogdanov, A.A., Vasiliev, V.D.
Format: Article
Language:English
Subjects:
5 S RNA
Biological and medical sciences
Cell structures and functions
Centrifugation, Density Gradient
Chemical Phenomena
Chemistry
Dinitrophenyl hapten
Electrophoresis, Polyacrylamide Gel
Endonucleases - metabolism
Escherichia coli - analysis
Ethylenediamines
Fundamental and applied biological sciences. Psychology
Haptens
Immune electron microscopy
Immunologic Techniques
Macromolecular Substances
Microscopy, Electron
Molecular and cellular biology
Nuclease S1
Ribosome
Ribosomes - analysis
RNA, Bacterial - analysis
RNA, Bacterial - metabolism
Single-Strand Specific DNA and RNA Endonucleases
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Summary:The internal site of 5 S RNA comprising residues A39 and U40 has been localized on the E.coli 50 S ribosomal subunit by immune electron microscopy. It has been found to be located on the interface side of the central protuberance at the position distinctly apart but very close to the position of the 5 S RNA 3'‐end providing evidence for a quite compact folded conformation of the 5 S RNA in situ.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80740-7