Histological analysis of the alterations on cortical bone channels network after radiotherapy: A rabbit study

The aim of this study was to evaluate the effects of radiotherapy in cortical bone channels network. Fourteen rabbits were divided in two groups and test group received single dose of 15 Gy cobalt‐60 radiation in tibia, bilaterally. The animals were sacrificed and a segment of tibia was removed and...

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Bibliographic Details
Published in:Microscopy research and technique 2010-10, Vol.73 (11), p.1015-1018
Main Authors: Rabelo, Gustavo Davi, Beletti, Marcelo Emílio, Dechichi, Paula
Format: Article
Language:English
Subjects:
Algorithms
Animals
Bone (cortical)
bone channels network
Bone matrix
Bone Matrix - radiation effects
Data processing
Histocytochemistry - methods
lacunarity
Microscopy
microstructure
Osteocytes
Osteocytes - radiation effects
Photomicrography
Rabbits
Radiation
Radiation Dosage
Radiotherapy
Radiotherapy - adverse effects
Standard deviation
Statistics, Nonparametric
Tibia
Tibia - cytology
Tibia - radiation effects
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Summary:The aim of this study was to evaluate the effects of radiotherapy in cortical bone channels network. Fourteen rabbits were divided in two groups and test group received single dose of 15 Gy cobalt‐60 radiation in tibia, bilaterally. The animals were sacrificed and a segment of tibia was removed and histologically processed. Histological images were taken and had their bone channels segmented and called regions of interest (ROI). Images were analyzed through developed algorithms using the SCILAB mathematical environment, getting percentage of bone matrix, ROI areas, ROI perimeters, their standard deviations and Lacunarity. The osteocytes and empty lacunae were also counted. Data were evaluated using Kolmogorov‐Smirnov, Mann Whitney, and Student's t test (P < 0.05). Significant differences in bone matrix percentage, area and perimeters of the channels, their respective standard deviations and lacunarity were found between groups. In conclusion, the radiotherapy causes reduction of bone matrix and modifies the morphology of bone channels network. Microsc. Res. Tech. 73:1015–1018, 2010. © 2010 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
1097-0029
DOI:10.1002/jemt.20826