Loading…

Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs

Hypercholesterolemia is characterized by a coronary vasoconstrictive response to the endothelium-dependent vasodilator acetylcholine. This abnormality may be due to reduced synthesis of endothelium-derived relaxing factor and/or enhanced synthesis and release of an endothelium-derived contracting fa...

Full description

Saved in:
Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 1993-12, Vol.88 (6), p.2923-2928
Main Authors: LERMAN, A, WEBSTER, M. W. I, CHESEBRO, J. H, EDWARDS, W. D, CHI-MING WEI, FUSTER, V, BURNETT, J. C
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c472t-bc6ab7f8834f7432d373ee2b706a1494652f0d0fdb0bc8b75aae205f7c211fcf3
cites
container_end_page 2928
container_issue 6
container_start_page 2923
container_title Circulation (New York, N.Y.)
container_volume 88
creator LERMAN, A
WEBSTER, M. W. I
CHESEBRO, J. H
EDWARDS, W. D
CHI-MING WEI
FUSTER, V
BURNETT, J. C
description Hypercholesterolemia is characterized by a coronary vasoconstrictive response to the endothelium-dependent vasodilator acetylcholine. This abnormality may be due to reduced synthesis of endothelium-derived relaxing factor and/or enhanced synthesis and release of an endothelium-derived contracting factor. Endothelin is an endothelium-derived vasoconstrictor and mitogenic peptide that is present in normal plasma, and its circulating concentrations are elevated in disease states that are characterized by abnormal endothelium-dependent relaxation to acetylcholine. The current studies were designed to test the hypotheses that experimental hypercholesterolemia results in elevation of plasma and tissue endothelin immunoreactivity and that the abnormal acetylcholine-evoked coronary vasoconstriction in the hypercholesterolemic animals is associated with further elevation of plasma endothelin. Plasma concentrations and molecular forms of endothelin immunoreactivity were determined following 2% cholesterol diet for 4 months in pigs and during intracoronary acetylcholine administration. Second, we assessed the presence of endothelin in the coronary vascular wall by using immunohistochemistry. Hypercholesterolemia elevated plasma endothelin concentration and enhanced coronary artery tissue endothelin immunoreactivity. The endothelium-dependent vasodilator acetylcholine further increases plasma endothelin in hypercholesterolemia in association with coronary vasoconstriction. The predominant molecular form of endothelin in hypercholesterolemia is the biological active endothelin-1. This study suggests a role for endothelin as an early participant and a marker for the endothelial dysfunction in hypercholesterolemia as well as a participant in the atherogenic process.
doi_str_mv 10.1161/01.cir.88.6.2923
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76113541</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76113541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c472t-bc6ab7f8834f7432d373ee2b706a1494652f0d0fdb0bc8b75aae205f7c211fcf3</originalsourceid><addsrcrecordid>eNo9UFtLwzAYDaLMOX33ReiD-Naaa5M-SvEyGAxEn0OaJlukN5NW2L83Y2VPh-87Fw4HgHsEM4Ry9AxRpp3PhMjyDBeYXIAlYpimlJHiEiwhhEXKCcbX4CaEn3jmhLMFWAjMMIf5EmxL5_XUqNF1u0R1dTK6ECaTmK7ux71pXJe4tp263hulR_fnxkMSf_vDYLze940Jo_ERWqeTwe3CLbiyqgnmbsYV-H57_So_0s32fV2-bFJNOR7TSueq4lYIQi2nBNeEE2NwFTspRAuaM2xhDW1dwUqLijOlDIbMco0RstqSFXg65Q6-_51iC9m6oE3TqM70U5A8R4gwiqIQnoTa9yF4Y-XgXav8QSIojxtKiGS5_pRCyFweN4yWhzl7qlpTnw3zaJF_nHkVtGqsV5124SwjgjHCOPkHwGF7uw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76113541</pqid></control><display><type>article</type><title>Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs</title><source>EZB Electronic Journals Library</source><creator>LERMAN, A ; WEBSTER, M. W. I ; CHESEBRO, J. H ; EDWARDS, W. D ; CHI-MING WEI ; FUSTER, V ; BURNETT, J. C</creator><creatorcontrib>LERMAN, A ; WEBSTER, M. W. I ; CHESEBRO, J. H ; EDWARDS, W. D ; CHI-MING WEI ; FUSTER, V ; BURNETT, J. C</creatorcontrib><description>Hypercholesterolemia is characterized by a coronary vasoconstrictive response to the endothelium-dependent vasodilator acetylcholine. This abnormality may be due to reduced synthesis of endothelium-derived relaxing factor and/or enhanced synthesis and release of an endothelium-derived contracting factor. Endothelin is an endothelium-derived vasoconstrictor and mitogenic peptide that is present in normal plasma, and its circulating concentrations are elevated in disease states that are characterized by abnormal endothelium-dependent relaxation to acetylcholine. The current studies were designed to test the hypotheses that experimental hypercholesterolemia results in elevation of plasma and tissue endothelin immunoreactivity and that the abnormal acetylcholine-evoked coronary vasoconstriction in the hypercholesterolemic animals is associated with further elevation of plasma endothelin. Plasma concentrations and molecular forms of endothelin immunoreactivity were determined following 2% cholesterol diet for 4 months in pigs and during intracoronary acetylcholine administration. Second, we assessed the presence of endothelin in the coronary vascular wall by using immunohistochemistry. Hypercholesterolemia elevated plasma endothelin concentration and enhanced coronary artery tissue endothelin immunoreactivity. The endothelium-dependent vasodilator acetylcholine further increases plasma endothelin in hypercholesterolemia in association with coronary vasoconstriction. The predominant molecular form of endothelin in hypercholesterolemia is the biological active endothelin-1. This study suggests a role for endothelin as an early participant and a marker for the endothelial dysfunction in hypercholesterolemia as well as a participant in the atherogenic process.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/01.cir.88.6.2923</identifier><identifier>PMID: 8252706</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams &amp; Wilkins</publisher><subject>Acetylcholine - pharmacology ; Animals ; Arteriosclerosis - etiology ; Biological and medical sciences ; Cholesterol, Dietary - administration &amp; dosage ; Coronary Vessels - drug effects ; Coronary Vessels - physiopathology ; Disorders of blood lipids. Hyperlipoproteinemia ; Endothelins - blood ; Endothelins - metabolism ; Hypercholesterolemia - blood ; Hypercholesterolemia - etiology ; Hypercholesterolemia - physiopathology ; Immunohistochemistry ; Male ; Medical sciences ; Metabolic diseases ; Swine ; Swine, Miniature ; Vasoconstriction - drug effects ; Vasoconstriction - physiology</subject><ispartof>Circulation (New York, N.Y.), 1993-12, Vol.88 (6), p.2923-2928</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-bc6ab7f8834f7432d373ee2b706a1494652f0d0fdb0bc8b75aae205f7c211fcf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3855357$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8252706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LERMAN, A</creatorcontrib><creatorcontrib>WEBSTER, M. W. I</creatorcontrib><creatorcontrib>CHESEBRO, J. H</creatorcontrib><creatorcontrib>EDWARDS, W. D</creatorcontrib><creatorcontrib>CHI-MING WEI</creatorcontrib><creatorcontrib>FUSTER, V</creatorcontrib><creatorcontrib>BURNETT, J. C</creatorcontrib><title>Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Hypercholesterolemia is characterized by a coronary vasoconstrictive response to the endothelium-dependent vasodilator acetylcholine. This abnormality may be due to reduced synthesis of endothelium-derived relaxing factor and/or enhanced synthesis and release of an endothelium-derived contracting factor. Endothelin is an endothelium-derived vasoconstrictor and mitogenic peptide that is present in normal plasma, and its circulating concentrations are elevated in disease states that are characterized by abnormal endothelium-dependent relaxation to acetylcholine. The current studies were designed to test the hypotheses that experimental hypercholesterolemia results in elevation of plasma and tissue endothelin immunoreactivity and that the abnormal acetylcholine-evoked coronary vasoconstriction in the hypercholesterolemic animals is associated with further elevation of plasma endothelin. Plasma concentrations and molecular forms of endothelin immunoreactivity were determined following 2% cholesterol diet for 4 months in pigs and during intracoronary acetylcholine administration. Second, we assessed the presence of endothelin in the coronary vascular wall by using immunohistochemistry. Hypercholesterolemia elevated plasma endothelin concentration and enhanced coronary artery tissue endothelin immunoreactivity. The endothelium-dependent vasodilator acetylcholine further increases plasma endothelin in hypercholesterolemia in association with coronary vasoconstriction. The predominant molecular form of endothelin in hypercholesterolemia is the biological active endothelin-1. This study suggests a role for endothelin as an early participant and a marker for the endothelial dysfunction in hypercholesterolemia as well as a participant in the atherogenic process.</description><subject>Acetylcholine - pharmacology</subject><subject>Animals</subject><subject>Arteriosclerosis - etiology</subject><subject>Biological and medical sciences</subject><subject>Cholesterol, Dietary - administration &amp; dosage</subject><subject>Coronary Vessels - drug effects</subject><subject>Coronary Vessels - physiopathology</subject><subject>Disorders of blood lipids. Hyperlipoproteinemia</subject><subject>Endothelins - blood</subject><subject>Endothelins - metabolism</subject><subject>Hypercholesterolemia - blood</subject><subject>Hypercholesterolemia - etiology</subject><subject>Hypercholesterolemia - physiopathology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Vasoconstriction - drug effects</subject><subject>Vasoconstriction - physiology</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNo9UFtLwzAYDaLMOX33ReiD-Naaa5M-SvEyGAxEn0OaJlukN5NW2L83Y2VPh-87Fw4HgHsEM4Ry9AxRpp3PhMjyDBeYXIAlYpimlJHiEiwhhEXKCcbX4CaEn3jmhLMFWAjMMIf5EmxL5_XUqNF1u0R1dTK6ECaTmK7ux71pXJe4tp263hulR_fnxkMSf_vDYLze940Jo_ERWqeTwe3CLbiyqgnmbsYV-H57_So_0s32fV2-bFJNOR7TSueq4lYIQi2nBNeEE2NwFTspRAuaM2xhDW1dwUqLijOlDIbMco0RstqSFXg65Q6-_51iC9m6oE3TqM70U5A8R4gwiqIQnoTa9yF4Y-XgXav8QSIojxtKiGS5_pRCyFweN4yWhzl7qlpTnw3zaJF_nHkVtGqsV5124SwjgjHCOPkHwGF7uw</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>LERMAN, A</creator><creator>WEBSTER, M. W. I</creator><creator>CHESEBRO, J. H</creator><creator>EDWARDS, W. D</creator><creator>CHI-MING WEI</creator><creator>FUSTER, V</creator><creator>BURNETT, J. C</creator><general>Lippincott Williams &amp; Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931201</creationdate><title>Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs</title><author>LERMAN, A ; WEBSTER, M. W. I ; CHESEBRO, J. H ; EDWARDS, W. D ; CHI-MING WEI ; FUSTER, V ; BURNETT, J. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-bc6ab7f8834f7432d373ee2b706a1494652f0d0fdb0bc8b75aae205f7c211fcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Animals</topic><topic>Arteriosclerosis - etiology</topic><topic>Biological and medical sciences</topic><topic>Cholesterol, Dietary - administration &amp; dosage</topic><topic>Coronary Vessels - drug effects</topic><topic>Coronary Vessels - physiopathology</topic><topic>Disorders of blood lipids. Hyperlipoproteinemia</topic><topic>Endothelins - blood</topic><topic>Endothelins - metabolism</topic><topic>Hypercholesterolemia - blood</topic><topic>Hypercholesterolemia - etiology</topic><topic>Hypercholesterolemia - physiopathology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Vasoconstriction - drug effects</topic><topic>Vasoconstriction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LERMAN, A</creatorcontrib><creatorcontrib>WEBSTER, M. W. I</creatorcontrib><creatorcontrib>CHESEBRO, J. H</creatorcontrib><creatorcontrib>EDWARDS, W. D</creatorcontrib><creatorcontrib>CHI-MING WEI</creatorcontrib><creatorcontrib>FUSTER, V</creatorcontrib><creatorcontrib>BURNETT, J. C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LERMAN, A</au><au>WEBSTER, M. W. I</au><au>CHESEBRO, J. H</au><au>EDWARDS, W. D</au><au>CHI-MING WEI</au><au>FUSTER, V</au><au>BURNETT, J. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>88</volume><issue>6</issue><spage>2923</spage><epage>2928</epage><pages>2923-2928</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>Hypercholesterolemia is characterized by a coronary vasoconstrictive response to the endothelium-dependent vasodilator acetylcholine. This abnormality may be due to reduced synthesis of endothelium-derived relaxing factor and/or enhanced synthesis and release of an endothelium-derived contracting factor. Endothelin is an endothelium-derived vasoconstrictor and mitogenic peptide that is present in normal plasma, and its circulating concentrations are elevated in disease states that are characterized by abnormal endothelium-dependent relaxation to acetylcholine. The current studies were designed to test the hypotheses that experimental hypercholesterolemia results in elevation of plasma and tissue endothelin immunoreactivity and that the abnormal acetylcholine-evoked coronary vasoconstriction in the hypercholesterolemic animals is associated with further elevation of plasma endothelin. Plasma concentrations and molecular forms of endothelin immunoreactivity were determined following 2% cholesterol diet for 4 months in pigs and during intracoronary acetylcholine administration. Second, we assessed the presence of endothelin in the coronary vascular wall by using immunohistochemistry. Hypercholesterolemia elevated plasma endothelin concentration and enhanced coronary artery tissue endothelin immunoreactivity. The endothelium-dependent vasodilator acetylcholine further increases plasma endothelin in hypercholesterolemia in association with coronary vasoconstriction. The predominant molecular form of endothelin in hypercholesterolemia is the biological active endothelin-1. This study suggests a role for endothelin as an early participant and a marker for the endothelial dysfunction in hypercholesterolemia as well as a participant in the atherogenic process.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams &amp; Wilkins</pub><pmid>8252706</pmid><doi>10.1161/01.cir.88.6.2923</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0009-7322
ispartof Circulation (New York, N.Y.), 1993-12, Vol.88 (6), p.2923-2928
issn 0009-7322
1524-4539
language eng
recordid cdi_proquest_miscellaneous_76113541
source EZB Electronic Journals Library
subjects Acetylcholine - pharmacology
Animals
Arteriosclerosis - etiology
Biological and medical sciences
Cholesterol, Dietary - administration & dosage
Coronary Vessels - drug effects
Coronary Vessels - physiopathology
Disorders of blood lipids. Hyperlipoproteinemia
Endothelins - blood
Endothelins - metabolism
Hypercholesterolemia - blood
Hypercholesterolemia - etiology
Hypercholesterolemia - physiopathology
Immunohistochemistry
Male
Medical sciences
Metabolic diseases
Swine
Swine, Miniature
Vasoconstriction - drug effects
Vasoconstriction - physiology
title Circulating and tissue endothelin immunoreactivity in hypercholesterolemic pigs
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A43%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Circulating%20and%20tissue%20endothelin%20immunoreactivity%20in%20hypercholesterolemic%20pigs&rft.jtitle=Circulation%20(New%20York,%20N.Y.)&rft.au=LERMAN,%20A&rft.date=1993-12-01&rft.volume=88&rft.issue=6&rft.spage=2923&rft.epage=2928&rft.pages=2923-2928&rft.issn=0009-7322&rft.eissn=1524-4539&rft.coden=CIRCAZ&rft_id=info:doi/10.1161/01.cir.88.6.2923&rft_dat=%3Cproquest_cross%3E76113541%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c472t-bc6ab7f8834f7432d373ee2b706a1494652f0d0fdb0bc8b75aae205f7c211fcf3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=76113541&rft_id=info:pmid/8252706&rfr_iscdi=true