Monoclonal antibodies to cytochrome P-450 immunopurify a 45-kDa protein from a human lymphoblastoid cell line

Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification c...

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Bibliographic Details
Published in:FEBS letters 1985-06, Vol.185 (1), p.67-70
Main Authors: Friedman, Fred K., Pastewka, Jullia V., Robinson, Richard C., Park, Sang Shin, Marietta, Michael A., Gelboin, Harry V.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Aryl hydrocarbon hydroxylase
Biological and medical sciences
Cell Line
Cytochrome P-450
Cytochrome P-450 Enzyme System - immunology
Cytochrome P-450 Enzyme System - isolation & purification
cytochrome P450
Enzyme Induction - drug effects
Enzymes and enzyme inhibitors
Epitope
Epitopes - immunology
Fundamental and applied biological sciences. Psychology
Humans
Immunologic Techniques
Immunopurification
Liver - enzymology
Lymphoblastoid cell
lymphoblastoid cell lines
Lymphocytes - enzymology
Methylcholanthrene - pharmacology
monoclonal antibodies
Monoclonal antibody
Oxidoreductases
Phenobarbital - pharmacology
Rats
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Summary:Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80742-0