Peripheral membrane molecules of leukocytes and NK cytotoxicity

Some leukocyte effector cell-surface molecules movement toward the adjoining target cells takes place during the reaction of NK cytotoxicity (NK R). The majority of the moving molecules are usually anchored via a divalent-ion-dependent interaction (PMM-M2+). The released PMM-M2+ can interact also wi...

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Bibliographic Details
Published in:Folia microbiologica 1993-01, Vol.38 (5), p.421-431
Main Authors: KUBRYCHT, J, MALIKOVA, P, HUAN, N. H, FISEROVA, A, BEZOUSKA, K, KRUZIK, P, STAJNER, K, MORAVEC, V, POSPISIL, M
Format: Article
Language:English
Subjects:
Antibody-Dependent Cell Cytotoxicity - physiology
Antigens, Surface - analysis
Biological and medical sciences
Cell Membrane - immunology
Cytotoxicity, Immunologic - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Killer Cells, Natural - immunology
Leukocytes - immunology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - immunology
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Summary:Some leukocyte effector cell-surface molecules movement toward the adjoining target cells takes place during the reaction of NK cytotoxicity (NK R). The majority of the moving molecules are usually anchored via a divalent-ion-dependent interaction (PMM-M2+). The released PMM-M2+ can interact also with the secreted tumor necrosis factor alfa (TNF-alpha). In agreement with PMM-M2+ movement, the number of TNF-alpha binding sites on the target cell surface increases during NK R. In addition, antibodies against PMM-M2+, as well as D-mannose- or N-acetyl-D-glucosamine-terminated oligosaccharides of PMM-M2+ inhibit NK R. A more detailed analysis of PMM-M2+ with monoclonal antibodies used flow cytometry and cell-surface biotinylation. Only 3 of 31 tested CD antigens (CD2, LAK-1 and CD45) were passed through this first strongly restricted experimental screening. The EDTA-released LAK-1 antigen, but not CD2 and CD45, interact with TNF-alpha and cell surface via a mannose-inhibitable interaction dependent on the presence of Ca2+ ions. The mechanism of possible participation of PMM-M2+ in cytotoxic events is discussed in relation to Ca2+ influx and subsequent cytolysin secretion.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02898770