Structure and organization of the gene encoding a mouse mitochondrial stress-70 protein

• Published in: FEBS letters 1993-12, Vol.336 (1), p.27-33
• Main Authors: Michikawa, Yuichi, Baba, Tadashi, Arai, Yuji, Sakakura, Teruyo, Kusakabe, Moriaki
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; C3H mouse strain-specific antigen (CSA); Carrier Proteins - genetics; DNA, Complementary; Exons; Fundamental and applied biological sciences. Psychology; Gene organization; Genes. Genome; Genomic DNA; Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins; Introns; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mitochondria - metabolism; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Peptide-binding protein74; Sequence Homology, Amino Acid; Stress-70 protein
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)81602-V

We have previously found that an antigenic protein specific for C3H strain mouse (C3H strain-specific antigen, CSA) is identical to peptide-binding protein 74 (PBP74). PBP74/CSA is a novel member of the stress-70 protein family in mitochondria. In this study, mouse genomic clones encoding PBP74/CSA, including the 5'- and 3'-flanking regions of the gene, have been isolated and sequenced. The PBP74/CSA gene contained 17 exons interrupted by 16 introns. Two dimeric repeats of the consensus sequence of the heat-shock element are present in the 5'-flanking region of the PBP74/CSA gene. Moreover, the first intron is interrupted within the amino-terminal leader sequence, the pattern of which is similar to that of cytochrome c 1, located in the mitochondria.