Spin probe clustering in human erythrocyte ghosts

A model has been developed for 5-nitroxide stearate, I(12,3), distribution in human erythrocyte ghosts which accurately predicts ESR spectral alterations observed with increased probe/total lipid (P/L) at 37 degrees C. This spin probe occupies a class of high-affinity, noninteracting sites at low lo...

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Bibliographic Details
Published in:The Journal of membrane biology 1985-01, Vol.84 (1), p.81-95
Main Authors: GORDON, L. M, LOONEY, F. D, CURTAIN, C. C
Format: Article
Language:English
Subjects:
5-nitroxide stearate
Biological and medical sciences
Biological membranes
Cyclic N-Oxides - metabolism
E.S.R
Electron Spin Resonance Spectroscopy
Erythrocyte Membrane
erythrocytes
Fundamental and applied biological sciences. Psychology
ghosts
Humans
man
Mathematics
Membrane physicochemistry
Molecular biophysics
Spin Labels - metabolism
spin probes
Tissue Distribution
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Summary:A model has been developed for 5-nitroxide stearate, I(12,3), distribution in human erythrocyte ghosts which accurately predicts ESR spectral alterations observed with increased probe/total lipid (P/L) at 37 degrees C. This spin probe occupies a class of high-affinity, noninteracting sites at low loading. Saturation occurs with increasing probe concentration, and, at higher loading, the probe inserts itself at initially dilute sites to form membrane-bound clusters of variable size. No 'low' probe remains at high P/L where all I(12,3) clusters in a 'concentrated' phase. This model allows determination of the dilute/clustered probe ratio, and shows that I(12,3) segregates in erythrocytes at what might otherwise be considered low P/L (e.g., 1/359). These findings validate the earlier use of empirical parameters to estimate probe sequestration in biological membranes.
ISSN:0022-2631
1432-1424
DOI:10.1007/BF01871650