Growth and plasmid‐encoded naphthalene catabolism of Pseudomonas putida in batch culture

The growth characteristics of Pseudomonas putida plasmid‐harbouring strains which catabolize naphthalene via various pathways in batch culture with naphthalene as the sole source of carbon and energy have been investigated. The strains under study were constructed using the host strain P. putida BS3...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-11, Vol.113 (3), p.303-307
Main Authors: Boronin, Alexander M., Filonov, Andrei E., Gayazov, Renat R., Kulakova, Anna N., Mshensky, Yuri N.
Format: Article
Language:English
Subjects:
Conjugation, Genetic - physiology
Degradation
Naphthalene
Naphthalenes - metabolism
Pathways
Plasmids
Plasmids - physiology
Pseudomonas
Pseudomonas putida
Pseudomonas putida - genetics
Pseudomonas putida - growth & development
Pseudomonas putida - metabolism
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Summary:The growth characteristics of Pseudomonas putida plasmid‐harbouring strains which catabolize naphthalene via various pathways in batch culture with naphthalene as the sole source of carbon and energy have been investigated. The strains under study were constructed using the host strain P. putida BS394 which contained various naphthalene degradation plasmids. The highest specific growth rate was ensured by the plasmids that control naphthalene catabolism through the meta‐pathway of catechol oxidation. The strains metabolizing catechol via the ortho‐pathway grew at a lower rate. The lowest growth rate was observed with strain BS291 harbouring plasmid pBS4 which controls naphthalene catabolism via the gentisic acid pathway. Various pathways of naphthalene catabolism appear to allow these strains to grow at various rates which should be taken into account when constructing efficient degraders of polycyclic aromatic compounds.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06531.x